Study Of The Expression Of Hepatoma-derived Growth Factor And P53 Protein In Colorrectal Cancer

Objective: To investigate the expression of HDGF and p53 protein in colorectalcancer tissues and their correlation with clinicopathologic factors.Methods: HDGF protein and p53 expression in colorectal cancer patients andnormal controls was detected by immunohistochemistry. SPSS statistical softwareprogram (version 11.5, SPSS, Inc.) was used for Statistical analysis.Result:(1) The positive expression rate of HDGF and p53 protein was 69.2% in 146 colorectal cancer samples and 67.8% in 59 colorectal cancer samples, respectively, while both of HDGF and p53 protein were undetectable in 10 normal controls of colorectal mucosal tissues.(2) By statistical analysis, HDGF expression was found to be associated with risk factors such as differentiated degree of colorectal cancer, lymph node metastasis and Dukes stage: the expression of HDGF in colorectal cancer of moderate and poorly differentiated adenocarcinoma, with lymph node metastasis and Duke's C and D was significantly higher than in colorectal cancer of well-differentiated adenocarcinoma, without lymph node metastasis, and Duke's A and B.(3) The total five-year survival rate was 42.5% in 146 cases of patients. The five-year survival rate after surgery in HDGF expression positive patients was 34.7%, compared with 60.0 % survival rate in HDGF expression negative patients, which had a significant difference (P <0.05). Multivariate analysis showed that HDGF was a indepentent prognostic factor for patients with colorectal cancer.(4) Recurrence and metastasis was found in 37(28.2%) of 131 patients with colorectal cancer received radical surgery, including 30 (34.1%) in 88 HDGF positive expression patients and 7 (16.3%) in 43 HDGF negative patients.(5)p53 expression was found to be associated with colorectal cancer lymph nodemetastases, The correlation coefficient was 0.282(P = 0.03).(6) By Spearman analysis, HDGF expression was associated with p53 proteinexpression in colorectal cancer. The correlation coefficient was 0.364 (P = 0.005).Conclusion:1 The expression of HDGF and p53 protein in colorectal cancer tissues was higherthan that in normal colorectal mucosal tissues. 2. HDGF expression was closely correlated with differentiated degree of colorectal cancer, lymph node metastasis , Dukes stage, recurrence and metastasis of colorectal carcinoma.3. Abnormal expression of HDGF was specifically found in colorectal cancer tissues, suggesting that HDGF may be used as a valuable prognostic factor for patients with colorectal cancer.4. p53 expression was found to be associated with colorectal cancer lymph node metastases5. HDGF expression was associated with p53 protein expression in colorectal cancer, suggesting that HDGF may paly a role in colorectal carcinogenesis, which warrant further studies.