| Objective:To observe the effect of Naoling Decoction on ethology, hippocamal histomorphology,and expression of GSK-3βand P-CREB in CA3 region of the rats with synthetic Alzheimer disease,and explore the therapeutical effect on Alzheimer disease rats and the potential mechanism of action of Naoling Decoction.Methods:Forty SD(spragu-dawleg)rats were classified into five groups after Y-electric-maze testing:normal group,sham- operated group, model group,Naoling Decoctio group and Piracetam group.The Alzheimer disease model was established by Aβ1—42 injected into hippocamal in rats. The faculty of learning and memory was evaluated by Y-electric-maze test and Morris water maze experiment after establishing AD model.The brain tissues were removed to be made frozen sections and stained with congored to detect the aggradation of Aβ,the changes of cell morphology were detected by HE stain and Nissl stain.Expression of GSK-3β,P-CREB in CA3 region was measured with immunohistochemical staining.RESULTS:(1)The results of Y-electric-maze test showed that compared with sham-operated group and normal group,model group's times to evade stimulate is more(p＜0.05);compared with model group,Naoling Decoctio group and Piracetam group's times to evade stimulate is less(p＜0.05). Meanwhile,there existed no obvious difference between these two therapy groups(p＞0.05).(2)The results of Morris water maze experiment showed that Compared with sham-operated group and normal group,the escaped and latent period of model group was delayed significantly(p＜0.05).In exploratory experiment,the average pass through times in sham operation group and normal group were more than that of other three ones,and that of the model group was lest,there were significant difference (p＜0.05).(3)Congored coloration result:Under the light microscope,in the hippocampal fields of rats in sham-operated group and normal group, orange amylaceous aggradation can't be seen,the cells in DG arrange in order. In the hippocampal fields of rats in the model group,significant orange amylaceous aggradation can be seen in the area that injecting Aβ1—42,this denota there is aggradation of Aβin the hippocampal fields of rats in the model group,meanwhile,the cells in DG obviously damaged,neuron loss noticeable,and there is neuroglial cell proliferation.(4)Under the light microscope,hippocampal CA3 fields of rats in the sham-operated group and normal group exhibited closely and neatly spaced pyramidal cells,and insignificant neuron loss;in the hippocampal CA3 fields of rats in the model group sparse and disturbed pyramidal cells,noticeable neuron loss and neuroglial cell proliferation could be seen,meanwhile,the number of pyramidal cells is less than sham-operated group and normal group(p＜0.05). Neuron loss reduced significantly in the hippocampal CA3 pyramidal cell fields of the two therapy group and cell morphology presented relative normal. (5)The result of GSK-3βwith immunohistochemical staining denote:The compasion of masculine cells about GSK-3βin each group of hippocampal CA3,the model one was the most,the Naoling Decoction group and Piracetam group were more than sham-operated group and normal group(p＜0.05).They had statistical significance.The compasion of the gray scale value about GSK-3βin hippocampal CA3 of each group,the model group were higher than sham-operated group and normal group(p＜0.05);the gray scale value of Naoling Decoction group and Piracetam group were lower than the model group's gray scale value(p＜0.05).(6)The result of P-CREB with immunohistochemical staining denote:The compasion of masculine cells about P-CREB in hippocampal CA3,of each group,the model one was the least,the Naoling Decoction group and Piracetam group were more than the model group(p＜0.05),They had statistical significance..The compasion of the gray scale value about P-CREB in hippocampal CA3 of each group,the model group were lower than sham-operated group and normal group (p＜0.05);the gray scale value of Naoling Decoction group and Piracetam group were higher than the model group's gray scale value(p＜0.05).CONCLUTIONS:(1)The presentation of results of the study that the model was established by Aβ1—42 injected into hippocamal was blest, because the model rats had the changes of praxiology and pathology.(2)The learning and memory of the model rats were decreased obviously,Naoling Decoction can improve the learning and memory of the model rats.(3)Naoling Decoction can dcrease neuronal loss and better pathomorphological changes in hippocampal CA3 of AD rats,thusly it has therapeutic action on vascular dementia rats.(4)Naoxintong capsule can weaken the expression of GSK-3βin hippocampal CA3 of AD rats,Elevate the expression of P-CREB,those partly may be therapeutic mechanism on Alzheimer disease of Naoling Decoction.
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