| Objective:To investigate the relationship of homocysteine,expression of gene GATA-4 and congenital heart disease.Methods:In our experiment we divided 30 pregnancy rats into three groups randomly:high dosage group,low dosage group and control group.From the 7thday of pregnancy,the high dosage group was injected intraperitoneally with HCY 200mg/(kg·d),the low dosage group was injected intraperitoneally with HCY 100mg/(kg·d),and the control group was injected intraperitoneally the same volume saline Sodium Chloride until the 20thday of pregnancy,when they were given uterine-incision delivery.Then we inspect the GATA-4 mRNA expression of a part of the neonatal rats' hearts by means of real time fluorescence quantify PCR. The result was delivered as Ct value and was analysed by SPSS 13.0 Statistic software.Results:GATA-4 mRNA of high dosage group expressed significantly more than that of low dosage rats(p＜0.05),and both of them showed significant difference with control group(p＜0.05).In high dosage group and low dosage group,GATA-4 mRNA of normal rats expressed significantly more than that of CHD rats(p＜0.05).GATA-4 mRNA of control group expressed significantly more than CHD rats(p＜0.05),but had no significant difference with heart-normal rats(p＞0.05).GATA-4 mRNA of hearts with ventricular septal defect(VSD)expressed significantly less than that with other types of CHD and normal hearts (P＜0.05).Conclusion:1.GATA-4 is an important transcription controlling factor during the development of heart,whose abnormal expression could lead to CHD.2.There was a dose-reaction relation between HCY and GATA-4,the more the HCY given to pregnancy rats,the less the GATA-4 expressed in neonatal rats' hearts.
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