| Fungi Pleurotus ostreatus(P.ostreatus)have many fine characteristics,like strongly reproductive Capacity,simple nutriments requirements,easy culture et al.Moreover,the P. ostreatus can produce abundant enzymes that can decompose traditional chinese medicine (TCM)and therefore promote releasing of the effective components from TCM.Thus,in the present work,we used P.ostreatus to ferment Codonopsis pilosula(C.piIosula).Firstly, spore was isolated from P.ostreatus for the preparation of seed.The fermentation condition was optimized with different concentrations of C.pilosula.Secondly,the compositions of fermentation broth were detected and analyzed.Thirdly,the effects of the fermentation broth on regulating the cellular immunity,anti-fatigue,anti-hypoxia,anti-thrombosis,analgesia and antagonizeing the sleep effect of Pentobarbital Sodium were investigated based on the traditional theories.The results showed that P.ostreatus growed well with 5%and 10%fermentation concentration of C.pilosula,it was found that the main medicinal compositions of C.pilosula changed after fermentation.The contents of saponin and flavonoid increased,but the contents of polysaccharide decreased.Both the species and contents of composition changed after fermentation by analyzing with HPLC.Compared with water decoction,animal experiments showed that the proper dose of fermentation broth could significantly prolong swimming time with load of mice and increase liver glycogen,prolong respiration time after decapitation, reduce the mice's twisting caused by acetic acid and enhance the pain threshold induced by heat irritation,prolong sleep latency and shorten sleeping time.However,there were no significant differences in the the mice's serum urea nitrogen content,blood lactate content, cellular immunity,the levels of lactic dehydrogenase hydrogen,the tolerance to lacking in oxygen under normal pressure,haemostasis,and coagulation between the fermentation broth and the water decoction treatments.
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