The Effect Of Exercise On The Expression Of Skeletal Muscle Fos And Heat Shock Protein 70 In Rats

Objective: To observe FOS and heat shock protein expression in rat skeletal muscle before, during and after different periods of acute and long-term endurance exercise, we try to identify in two different exercise circumstances whether FOS and heat shock protein have relativity or not.Methods: 100 male SD rats were randomly divided into two groups: acute and long-term endurance exercise group. As the incremental load running with Bedford treadmill running model and treadmill runing model of YunChang referenced, rats were killed accordance with points of design in experiment after last exercise. The change of skeletal muscle was viewed by HE dyeing. The expression of FOS and HSP70 were detected by immunohistochemistry stain. Using LEICA Qwin pictures analyze system measured the area of positive cell of FOS and HSP70 expression. The data were analyzed by SPSS 13.0.Results: (1) Activities of CK in serum in skeletal muscles of rats after acute exercise were significantly higher (P <0.05) after five hours, the movement reached a peak at 12 hours after exercise, and continued until 24 hours after exercise (P <0.05) . In the long-term endurance group, after 10 weeks of training three group have no significant difference.2) in the acute group, the rest control group have no positive cells, but in immediately after exercise group positive cells significantly increased (P <0.01), one hour after exercise the peak appeared (P <0.01), and continued until three hours after exercise, After five hours it has been gradually declining but still significantly higher than the quiet control group 24 hours after exercise (P <0.05). In the long-term endurance group, the group immediately after exercise positive cells and quiet control rest group was significant (P <0.05) after 24 hours positive movement returned to the level of quiet levels.(3) HSP70 in the acute exercise group, the control rest group, there are quiet a small number of positive cells, the movement of immediately after exercise group positive cells significantly increased (P <0.05), one hour after exercise group was further increased (P <0.01), the exercise of the 3 reached a peak hour, has continued to campaign after 5 hours, 24 hours after the campaign started to decline, but still significantly higher than the quiet control group (P <0.05). In the long-term endurance group, the Movement immediately after exercise group positive cells keep significantly increased (P <0.01) Until 24 hours after exercise.Conclusion: In this study we have come to conclusions (1) an acute movement caused rat gastrocnemius muscle fiber with disorder, muscle cells, such as Swelling and Congestive, while long-term endurance training rat gastrocnemius fiber is obviously enlarged, stromal cells reduce and the structure is more dense.(2)an acute movement can cause serum CK significant changes in rats at 24 h after exercise. But long-term endurance exercise after 24 h of serum CK has not changed. (3)Exercise can significantly induced c-fos and HSP70 expression in skeletal muscle of rats . sports injury can increased expression of HSP70 and c-fos. (4) FOS expression quickly after exercise it can reached a peak at 1 hour after exercise. HSP70 can reached a peak at 3 hour after exercise. FOS protein maybe induces the expression of HSP70 expression.