Protection On Impaired Intestine Mucosal Barrier By Bombesin In Rats With Obstructive Jaundice

ObjectiveTo investigate the protection on impaired intestine mucosal barrier by bombesin in rats with obstructive jaundice.MethodsForty male Wistar rats were randomly divided into four groups: I ,controls; II ,sham-operated; III ,bile duct ligation(BDL);and IV,BDL+Bombesin(BBS) (30μg/kg/d).At the end of the experiment,on day 10, the quantitative bacterial evaluation of intra-abdominal fluid from each animal was performed , endotoxin was measured in blood ,the total bilirubin and ALT were determined and compared ,the bacterial culture of the blood and mesenteric lymph nodes was performed .Meanwhile , liver tissue samples were examined histologically and Terminal ileum mucosa was observed under the electron microscope.ResultsThe value of endotoxin in group IV was significantly lower than that in group III((129.58±13.79)pg/ml vs (239.03±32.0)pg/ml,p<0.05 ) , The value of total bilirubin in group III was significantly higher than that in group II, The value of ALT in group IV was significantly higher than that in group III,the positive rate of bacterial culture of the blood and the mesenteric lymph nodes in group IV was significantly lower than that in group III(p<0.05), quantitative cultures of intra-abdominal fluid demonstrated higher bacterial numbers in group IV than in group III(113.67±8.0CFU/ml vs 363.19±15.1CFU/ml,p<0.05). The neutrophil infiltration and hepatic cellular swelling were significantly decreased in group IV than in group III. The terminal ileac mucous membrace got thicker and the villi got longer and stronger in group IV than in groupConclusionBombesin has significant effect on protecting the injuried mucosa barrierin obstructive jaundice , and can decrease endotoxemia and bacteriatranslocation.