| Objective To study renal pathological changes in senescence accelerated mouse P8 (SAMP8) , to investigate protective effects of ginsenoside Rb1/Rg1 on aging kidney of SAMP8 and its underlying mechanism.Method SAMP8 (9 months) were used as senescence accelerated animal model and senescence accelerated mouse/resistance 1 (SAMR1) with same age as a control group. Aging kidney was determined by several senescent indicators, such as appearance and behavior, PAS and Masson staining, SA-β-galactosidase activity measured by histochemistry staining and extracellular matrix components (fibronectin and collagen III) measured by immunohistochemical staining. Then 6-month-old SAMP8 mice were randomly divided into seven groups: one SAMP8 group and six Ginsenoside Rb1/Rg1 groups (treated with dosage of 2.5, 5, or 10 mg/kg/d by consecutively intraperitoneal injection).All eight groups (together with SAMR1 group) were feed in SPF (specific pathogen free) conditions and lasted three months. Then senescent indicators were compared among all eight groups and the expressions of renal fibronectin,collagen III,TGFβ1,BMP-7 were also measured by immunohistochemical staining to investigate protective effects of ginsenoside Rb1/Rg1 on aging kidney of SAMP8 and its underlying mechanism.Results Compared with SAMR1 group, SAMP8 group appeared different levels of senescence. Renal pathological structural changes, including tubulointerstitial fibrosis and focal segmental glomerulosclerosis were found through microscope,and it is significantly injury in tubulointerstitial. Positive staining for SA-β-galactosidase was widely observed. And the expression of fibronectin and collagen III increased remarkably,indicating tubulointerstitial fibrosis. Compared with SAMP8 group, ginsenoside Rb1/Rg1 could improve all senescent indicators as above to some degree, and the group treated with 5 mg/kg/d Rb1 and with 10 mg/kd/d Rg1 showed the most effective protection. Meanwhile, ginsenoside Rb1/Rg1 could significantly decrease the level of TGFβ1 and up-regulate BMP-7. The group treated with 5 mg/kg/d Rb1 and with10 mg/kd/d Rg1 were most significant.Conclusion Renal pathological changes in SAMP8 mice can be used for a model of aging kidney. Ginsenoside Rb1/Rg1 may exert a protection on aging kidney of SAMP8. Groups with 5 mg/kg/d Rb1 and with10 mg/kg/d Rg1 were most significant ones. Ginsenoside Rb1/Rg1 could reduce the abnormality accumulation of renal ECM to delay the renal fibrosis, probably through inhibiting the expression of TGFβ1 and up-regulate BMP-7.
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