| Objective:To study relation between protein tyrosine phosphatase 1B(PTP1B) with obesity,to compare PTP1B expression in obese individuals and in lean individuals,and to compare PTP1B expression between omental and subcutaneous adipose tissue.Method:To assay waist and hip circumference,fasting blood glucose and insulin concentrations,2-h postprandial glucose concentrations,fasting lipid profiles(total cholesterol,HDL-cholesterol,LDL-cholesterol,and total serum triglycerides) in 51 persons(9 men and 42 women;age range,15～39 years),including 10 lean[body mass index(BMI) 18.5～25kg/m~2]as control subjects(group C) and 41 obese individuals divided into two groups,16 for group A(BMI≥40kg/m~2) and 25 for group B(BMI 30～39.9kg/m~2).Adipose tissue samples were obtained within 30 minutes of the beginning of the surgery from the abdominal subcutaneous wall and from the greater omentum.To homogenize the adipose tissue samples and to extract protein of them.To assay and unify the protein concentration in the homogenates.The expression of PTP1B in their omental and subcutaneous adipose tissue was assayed by western blotting.Result:There's no statistical difference in age between objects in each group. Expression of PTP1B in both omental and subcutaneous adipose tissue of group A and group B were at higher levels than that of group C.The omental adipose tissue had a higher level of PTP1B expression level than subcutaneous adipose tissue in both group A and group B,though there's no statistical difference in PTP1B expression between the two depots of group C.In addition,the expression of PTP1B in omental adipose tissue was associated with BMI,HOMA-IR and ISI in group A and group B.Conclusion:Over-expression of PTP1B is involved in the obesity and the associated insulin resistance.Insulin resistance is related to omental adipose tissue more than subcutaneous adipose tissue,which plays more important role in obesity and associated co-morbidities. |
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