| Objective:To explore the significance of surfactant proteins expression in the development of coal workers' pneumoconiosis by measuring SP-A & D levels in serum and whole lung lavage fluid,which was retrieved by whole lung lavage on coal-miners with suspected and diagnosed simple coal workers' pneumoconiosis(0+ stage andâ… stage CWP),and the association of surfactant proteins expression with the deterioration of crucial lung function and blood gas parameters,and to evaluate both SP-A and SP-D to be used as susceptible biomarkers in predicting the development of coal workers' pneumoconiosis.Methods:15 coal miners diagnosed as 0+ stage as well as 17 patients asâ… stage coal workers' pneumoconiosis were included in the present study whose occupational coal dust exposure history was 5-35 years respectively.All of the subjects were male and their age was 36-54 years old and 47 years old on average.Whole lung lavage was conducted on them under venous anaesthesia condition,and the whole lung lavage fluid was recovered in conventional way.The peripheral venous blood was collected from those subjects and the blood gas and lung function parameters were also measured at mean time.The concentration of SP-A and SP-D in both whole lung lavage fluid and serum were determined with commercial ELISA kits respectively according to the protocol described by manufactory.Results:1 The concentration of SP-D from both whole lung lavage fluid and serum were statistically significant elevated in the patients withâ… stage CWP compared to that in coal miners with 0+ stage CWP(601.86±450.62pmol/L and 56.43±8.54nmol/L vs 280.76±152.50pmol/L and 44.45±16.18nmol/L,P＜0.05 respectively).2 A statistically significant negative correlation was found between the concentration of SP-A in serum and DLco%(r=-0.603,P＜0.05).3.The value of PO2(A-a) showed significantly higher inâ… stage CWP patients than that in 0+ stage CWP coal miners(10.5±4.41mmHg vs 3.20±1.48mmHg,P＜0.01).Conclusion:The present study indicated that the expression of SP-D both in whole lung lavage fluid and serum of 15 0+ stage and 17â… stage CWP coal miners were closely associated to the progress of pneumoconiosis.The level of serum SP-D in patients withâ… stage coal workers' pneumoconiosis was significantly elevated compared to that in 0+ stage patients,and serum SP-A concentration was found to significantly correlated to DLco%-a crucial index of lung fibrosis.The results above made it possible to use serum SP- D and SP- A as sensitive biomarker for evaluation of lung inflammation and fibrosis in coal miners more conveniently than BALF or something else.PO2(A-a),in our study,seemed to be more susceptible than DLco%in response to coal dust exposure.
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