Study On The Interrelationship Of LGT And The Prognosis Of Patients With Diffused Large B Cell Lymphoma

Objective: To screen the LGT in patients with diffused large B cell lymphoma(DLBCL) by surface-enhanced laser desorption/ionization-time of flight mass spectrometry(SELDI-TOF-MS) and further study the interrelationship of it with the prognosis of patients.Methods: we screened 62 serums sample of DLBCL by SELDI-TOF-MS and weak cation exchanger(WCX2) protein chip,and detected their LDH-level by biochemical measurement,β2-MG and CA125 level by ELISA.The LGT protein were analyzed between normal and the DLBCL patients who were in remission stage,replased and worse condition.The time survival and the log-rank test were used to statistical analysis.The correlation analysis with pretein spectrometry, LDH,β2-MG and CA125 were analyzed with spearman.Results: (1) The protein profiles revealed that a prominent peaks with the LGT were significantly increased in 20 DLBCL patients who were in replased,worse condition or before they died.These patients'survival time were significantly shorter than whom LGT peaks were not increased(p<0.01) .(2) LDH,β2-MG and CA125 in LGT positive group were higher than that in negative group(P<0.05),andβ2-MG and CA125 were positive correlation with LGT(p<0.01).(3)The patients'survival time in IPI low or middle group whose LGT peaks were shorter than whom LGT were negative (p<0.01) .(4) LGT were significantly increased in refractoriness group than easy group, consequence have statistical significance. (χ2=10.4,p=0.001)(5) The LGT companied peaks in 6107,7974,8067 were increased,and these peaks were positive correlation with LGT(p<0.01) .Conclusion: The appearance of LGT protein can be the specific worse prognosis biomakers for DLBCL patients.