Relationship Between Apoptosis With MCM4 And CDC6, CDK1, TFF2 And HPV_16/18 Infection In Uterine Cervical Carcinogenesis

Objective : To investigate the relationship between apoptosis with MCM4 and CDC6,CDK1,TFF2 and HPV16/18 infection in uterine cervical carcinogenesis.Methods:Formalin-fixed, paraffin-embedded samples including 50 cases of squamous cell carcinomas, 20 cases of cervical intraepithelial neoplasia CINⅡ-Ⅲand 20 cases of CINⅠand 20 cases of normal cervical tissues were studied. Using immunohisto-chemical technique (PV-9000), the authors examined the expression of MCM4, CDC6, CDK1and TFF2 in these samples. The infections of HPV type 16, 18 DNA were determined by PCR. The data of this study was analyses usingχ2 test,Nonparametric Tests and Spearman rank correlation.Results:(1) There were significant differences for MCM4, CDC6,and CDK1 between carcinomas, CIN and normal cervices, respectively (all P<0.05). Expression of MCM4, CDC6and CDK1 were correlated with tumor grades(all P<0.05). Expression of MCM4 and CDC6 were correlated with lymphnode metastasis (all P<0.05). Expression of MCM4, CDC6 and CDK1 were not correlated with age group and clinical stages (all P>0.05).The positive expression of TFF2 in carcinomas was significantly lower than in normal cervical tissue and CIN , there was significant differences among them ( P<0.05 ). The positive rate of TFF2 was associated with age group ( P<0.05 ).The positive rate of TFF2 was not associated with tumor grades, clinical stages and lymph node metastasis(all P>0.05).(2) The positive rate of HPV16/18 was significant difference between cervical carcinomas, CIN and normal tissues (P<0.05) and was not associated with age group, tumor grades, clinical stages and lymph node metastasis (all P>0.05).(3) The expression of MCM4 and CDK1 showed positive being correlated with the expression of CDC6 (rs=0.390 ,P<0.05; rs=0.529 ,P<0.05). The positive rate of HPV16/18 showed respectively positive being correlated with the expression of MCM4 and CDC6(rs=0.634,P<0.05; rs=0.386,P<0.05). MCM4 ,CDC6 and HPV16/18showed respectively negative correlation with the expression of TFF2(rs=-0.375, P<0.05; rs=-0.356, P<0.05;rs =-0.500 ,P<0.05)。Conclusions:The results suggest that in tissues of cervical carcinomas changes in MCM4, CDC6 and TFF2 expression may be associated with HPV16/18 infection and they interact with each other, which can influent the progression of CIN and carcinogenesis of cervical carcinomas. HPV16/18 may be an important role but not only increases the occurrence of cervical carcinomas .Expressions of MCM4, CDC6, and CDK1 are associated with uterine cervical carcinogenesis. They possibly become useful indexes for the primary screening and prognosis of cervical carcinomas.