| Objective: Tissue defect is a common clinical surgery problem, some serious defect need to use substitutes for filling and repair. Nano- hydroxyapatite (HA) as a bone substitute has aroused extensive attention and is one of important study topic in the field of biological materials around the world. Many studies have confirmed the results of Hydroxyapatite are a non-toxic, non-carcinogenic effects and good biocompatibility of biomaterials. Polytetrafluoroethylene (PTFE) molecular structural stability, it's has been used more than 40 years for clinical application. It has good biocompatibility, and has no carcinogenic and mutagenic. PTFE can be safe replaced Silicone (SI) as implantation of the first option. On plastic surgery, implants were replaced in the organization must have good biocompatibility, and the implants should get close with organization and do not have change shape. HA for human skeleton inorganic components, have good biocompatibility but fatal weakness is hardly forming and absorbed by organizations cause changes in shape. PTFE has good biocompatibility but the implant will produce coated so that can not get very close with organization, and could still feel minor foreign body sensation. Will optimize the combination of the two, interact in a synergistic way? To that end, after repeated study, synthetic nano- hydroxyapatite PTFE composite, on the one hand retain the biological characteristics of hydroxyapatite, on the other hand with the biological properties of PTFE. This study aims to assess and PTFE nano- hydroxyapatite composite biocompatibility.Methods: Healthy adult SD rats of either sex, weighing 120 g to 150 g were randomly divided into two groups, namely, the experimental group (HA + PTFE) and the control group (SI). SD rats be used ether to anesthesia,in the rat calvarial bone subperiosteal lacunar separation be filled with HA and PTFE. To separate Left hind leg muscle and skill filled with HA and PTFE. Use this method, such as silicone rubber prosthesis volume at the same location. Completed animals will be sent back to observation. 15, 30 and 60 days respectively in the animals were killed, physical and chemical examination.Detection:(1) Observation of specimen: incision near the organization, and the surrounding tissue graft, the combination of graft rejection in there.(2) X-ray inspection: To observe the complex silicone prosthesis and the combination of the situation and the organization.(3) Histological examination: In the light microscope observation of the surface in the graft and internal organization changes.(4) Determination of new bone area: high-power light microscope every measurement of the mirror vision of the group of new bone, spss software used for data analysis.(5) Graft coating thickness of the fiber membrane.Results:1. SI may have physiological inert plastic, minimal tissue reaction characteristics in vivo after implantation does not cause foreign body reaction, but it will surface in the graft will be formed a thick layer of connective tissue envelope, lead close contact with organizations in Mobile.2. HA + PTFE composite along with the advantages of the two materials, HA can be combined with the host bone to graft does not move, and because of the graft to join PTEF morphological changes will not happen, the complexes have better biocompatibility, not ectopic bone. Conclusion: HA and PTFE have good biocompatibility, is a more ideal human tissue filler material. HA mixed with PTFE has strong hand, and even before the complex extent by changing the HA, PTFE complex in the proportion of the adjustment, improve the complex granular HA simply do not have plasticity and can be gradual degradation organizations shortcomings. Such complexes could be the same with the HA in the body and guide the formation of bone tissue joint bone tissue and in bone tissue within organizations outside the induction of bone tissue. HA and PTFE composite and silica gel is better than the biocompatibility. |
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