Empirical Study Of Therapeutical Effect Of DBJN To Endometriosis

ObjectiveDBJN is a 3^rdnew drug of state.It consists of danshen root,Panax pseudo-ginseng, sparganii-rhizoma,zedoaria,peach seed(decorticate),angelica,carapax amydae,kelp, eucommia(parched by salt),atractylodes macrocephala(parched),scutellariae barbatae-herba and ramulus cinnamomi.Effectivenesses of it are activating blood circulation to dissipate blood stasis and soften hard lumps and dispel nodes.It is used to threat endometriosis,blocks of pelvic inflammatory disease,which show pain of hypogastrium and waist,excessive fluor,tenesmus of anus and dark red texture of tongue, caused by qi-stagnancy and blood stasis.In our study,we observed effectiveness of DBJN in rats with endometriosis by blood rheology,antiinflammatory,relieve pain,anti-thrombus and so on.2Methods and Results2.1 Effects on rats with endometriosisTo build endometriosis models on S rats(♀)and treated them for 30 days.And then PGE2 and TNF in blood and peritoneal exudates of rats were detected by RIA.Transpls of endometrial were observed by vivo and microscope.Results show E2 and corporin in serum and PGE2 and TNF in serum and peritoneal exudates of rats,volum and weight of transpls of endometrial were reduced by different doses of DBJN compared with the control group,P＜0.05～0.01.Pathohistology of these transpl shows more poor cystogenesis,expansion of glandular cavity and epitheliogenetic level in treated groups than in control group.2.2Anti-inflammatory effect2.2.1 Effects to cotton ball granulomaModel of cotton ball granuloma was build on S rats(♂).After successive administration for 6 days,rats were killed and cotton ball granulomas were cut out to be weighted in humid weight and dry weight.Results show humid weight and dry weight of cotton ball granulomas are lower in high and middle dose of DBJN than in control group,P＜0.01. 2.2.2 Effects to arthrophlogosis caused by carrageenanModel of arthrophlogosis caused by carrageenan on S rats(♂)after successive administration for 6 days.And diameter of left arthrals of these rats were measured before models were built and 1 hour later by sliding caliper.Results show diameter reduced more in high,middle and low dose of DBJN than in control group,P＜0.01.2.3 Analgesic effect2.3.1 Effects to pain caused by HAcNIH mice(♀&♂)successively administrated for 3 days and HAc was used to cause pain. And the starting time and times of writhing response in 10 minutes of these mice were observed.Results show the high,middle and low dose of DBJN can more prolong the time of writhing response starting and reduce the times than in control group,P＜0.01.2.3.2 Effects to pain caused by heatQualified NIH mice(♀)who licked their own metapedes in 30s were choosed to be successively administrated for 3 days by intellective hot plate(YLS-6B).And pain thresholds of 30,60 and 90min of these mice after the last administration were measured by hot plate.Results show high and middle dose of DBJN can more elevate pain thresholds of these mice in 30 and 60 min than the control group,P＜0.05～0.01.However,there is no differences compared with the group of Guizhifuling-capsule,P＞0.05.2.4 Effects of promoting blood flow2.4.1 Effects of auricle microcirculation disturbance of mice model of depression of liver-QI and cold coagulation- blood stasisNIH mice(♀&♂)successively administrated for 18 days and model of depression of iiver-QI and cold coagulation- blood stasis was built for more 2 days(administration continued).One hour after the last administration,change of blood flow rate of auricles of these mice was measured by laser-Doppler circulation system.Results show blood flow rate of auricles is quicker in high,middle and low dose of DBJN than in control group, P＜0.01.2.4.2 Effects of venothrombotic in cava of mice model of depression of liver-QI and cold coagulation- blood stasisS rats(♀&♂)successively administrated for 18 days and model of depression of liver-QI and cold coagulation- blood stasis was built for more 2 days(administration continued). One hour after the last administration,venothrombotic was build in cava.And venothrombotics were observed in cava and humid weight and dry weight of these venothrombotics to be weighted by electronic balance.Results show humid weight and dry weight of venothrombotic are lower in high and middle dose of DBJN than in control group, P＜0.01.2.4.3 Effects to haemorheology of rats with depression of liver-QI and cold coagulation- blood stasisS rats(♀&♂)successively administrated for 13days and model of depression of liver-QI and cold coagulation- blood stasis was built for more 2 days(administration continued). One hour after the last administration,getting blood from vena orbitalis and measuring blood viscosity by blood rheometer(PRECIL LBF-N6B).Results show blood viscosity was more depressed by high,middle and low dose of DBJN and plasma viscosity,too, compared with control group,P＜0.05～0.01.3 ConclusionDBJN can be used to treat endometriosis of rats by reducing PGE₂ and TNF in blood serum and peritoneal fluid,anti-inflammatory,analgesic,improving rheological characteristic and so on.The results show features of Chinese medicine of multicomponent and multi-effect.