| Objective: To investigate the effect of green tea polyphenols and acupuncture on the behavior and the dopamine level of paraquat induced Parkinson's disease mice model, and to explore the protection and its mechamism in those PD mice model. Methods: Eight-week-old male C57 mice were divided into 5 groups randomly: negative control, paraquat, green tea polyphenols, acupuncture,green tea polyphenols and acupuncture, dose of 10mg/kg paraquat exposed to all the groups except negative control. And with the exception of the negative control and paraquat group, the others treated with protective medium. The changes of behavior were observed at different periods with pole-test, to determine whether those mice have PD symptom. After 8 weeks, the mice were killed with decapitation, the changes of neurons of the substantia nigra in midbrain were observed with HE staining, And the level of dopamine in brain was measured with HPLC-UV. Results: The symptoms like tremor, piloerection, raising,ante-limb,slow-moving,and aberrant behavior in paraquat group were more obviously than others. Compared with control, the score of praxiology in paraquat group had statistical significance(P<0.05); but the appearance time of such symptoms and pole-test time had no statistical significance(P>0.05). Pathological changes showed that, dopaminergic neurons decreased and density losed obviously in paraquat group compared with control, but obvious differences were not found among protective additive groups. Compared with control group, the level of DA decreased and obviously in paraquat with HPLC-UV, but the difference had no statistical significance (P>0.05). Conclusion: The tests prompt that PQ can make C57 mice appear Parkinsonism symptom and decrease dopaminergic neurons in substantial nigra, and the process of the disease is chronic. Green tea polyphenols and acupuncture really improve the behavior of paraquat injury C57 mice, but the mechanism needs further investigation.
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