Effects Of Different Means Of Resuscitation On TNF-α,IL-6 And Visceral Organ Function In Rats With Abdominal Infection After Hemorrhagic Shock

Objective: To observe the effects of different means of resuscitation on TNF-α, IL-6 and main visceral organs function in rats with abdominal infection after hemorrhagic shock. Methods: Rat models of hemorrhagic shock were reproduced using the modified Capone methods.Fourty–six male SD rats were randomly divided into two groups:Limited fluid resuscitation group, large quantity fluid rapid resuscitation group(each n=23). Using the different means of resuscitation .Then each group were randomly divided into two different time points abdominal injection of LPS (LPS=2mg∕kg), or 4 hour and 8 hour when the MAP≥80mmHg and HCT≥30%, measured the contents of TNF-α,IL-6 with ELISA and determined 8 hour heart, the liver, kidney's biochemistry target. Results: The contents of TNF-αand IL-6 of the limited fluid resuscitation group was decreased than the large quantity fluid rapid resuscitation group at the same time(P<0.05). The 8 hour heart, the liver, kidney's biochemistry target in limited fluid resuscitation was significantly better than that in large quantity fluid rapid resuscitation group(P<0.05).Conclusion: The limited fluid resuscitation could obviously reduce the blood loss and mortality, while secondary recovery after abdominal infection, limited fluid resuscitation can reduce the rats TNF-α, IL-6 levels and the heart, liver, kidney inflammation response...