EGCG Effect Of IVM, IVF And Embryo Development On Mouse

In vivo, tissue oxidation metabolic process generates reactive oxygen species (ROS), which is the generic name of superoxide anion(O2-), hydrogen dioxide(H2O2), hydroxy radical(HO-), nitrogen monoxidum(NO-) etc..ROS participates pHysiologic,adjustment nerve internal hormone, circulation and immune, priming multiple factor biological effect in cell poikilostasis and others vital movement as a second messenger. This newly discovered signal molecule's chemical property is so active that it would easily initiate xidation-reduction reaction. Under damage conditions with more oxidation metabolins generated, or when anti-oxidation protection is deficient in cell, ROS accumulates and poisons cell. In vivo, oxygen radicals'damage is prevented or limited by antioxidants(or scavengers of free radicals), including superoxide dismutase(SOD), catalase, selenium-dependent glutathione peroxidase (SeGPX) and some lipid or water soluble Vitamins such as VC, VE. However, antioxidant level in vitro culture of oocyte and embryo is lower than in vivo development, that's because oocyte and embryo could not benefit from the maternal antioxidant protection when divorced from the donor body. This is a restrict factor in vitro culture. In our experiment we added antioxidant to optimize vitro culture circumstances. EGCG is a natural antioxidant extracted from tea, having no toxico-side effect. In this study, we used mouse as animal model, deployed HTF and TCM-199 as foundation culture systems, added different levels of EGCG to study EGCG effect on IVM, IVF and embryo development. Mechanism of EGCG action was approached by determining glutathione(GSH) in matured oocyte, fundamental findings described as follows:1.G1/G2 culture solution was better than HTF and M16 for mouse in vitro development.2.In TCM-199 system, when added 20μM EGCG, COCs had maxlmum maturing rate ,fertility rate and embryonic development rate.3. In TCM-199 system, when added EGCG, EGCG had no effect on DO, it could not elevate maturing rate, ertility rate and embryonic development rate,overdose EGCG could significantly degrade maturing rate, fertility rate and embryonic development rate.4.In HTFsystem, when added 20μM EGCG, COCs had maxlmum maturing rat, fertility rate and embryonic development rate.5.In HTF system,when added EGCG, EGCG had no effect on DO, it could not elevate maturing rate, fertility rate and embryonic development rate,overdose EGCG could significantly degrade maturing rate, fertility rate and embryonic development rate.6.When added 20μM EGCG, matured oocyte(COCs)had maxlmum GSH,but no effect on DO.7.When added 25μM EGCG, GSH in matured oocyte(COCs)was significantly degraded.8.By contrast, HTF cultivante resulted better than TCM-199.