Expression And Activation Of TAK1 Gene In Synovial Tissues Of Rheumatoid Arthritis

IntroductionRheumatoid arthritis (rheumatoid arthritis, RA) is a chronic autoimmune disease, to joint synovitis and bone symmetrical joints,cartilage destruction as the main feature. The etiology of rheumatoid arthritis is not clear,but we know that a variety of inflammatory cytokines play an important role in the pathogenesis of RA. Transforming growth factor-βactivation of protein kinase-1 (TAK1,MEKK7), which is a member of the MAP3K family and an important kinase in the cell signal of inflammatory response, may play an important role in the incidence of RA.Materials and MethodsMaterials1.23 patients with knee joint synovial tissues from the First Affiliated Hospital of China Medical University orthopedic. Of these, 10 cases of patients with rheumatoid arthritis, 7 cases of osteoarthritis patients, 6 cases of patients without abnormal by exploration of knee arthroscopy.2.Reagents for RT-PCR3.Reagents for Western blotMethods1. RT-PCR was used to test the changes of TAK1 mRNA level in the case group and the control group.2. Western blot was performed to test the TAK1 protein level in the case group and the control group.3. Western blot was performed to test the difference of TAK1 kinase activation. ResultsThere was a statistically significantly higher mean TAK1 mRNA, protein expression and kinase activity in the knee joint synovial tissue of patients with RA vs patients with OA and with no abnormal.ConclusionThese data are the first to demonstrate the association of TAK1 with RA and hence provide a biological marker and a useful prognostic indicator for RA. It is useful for diagnosis and therapies of RA.