| PrefaceFA gene was founded in Fanconi anemia(FA).At present,it was been confirm to have 11 subtyps(such as A,B,S,D1,D2,E,F,G,I,J and 1),now,it is draw offthat:FA roles on the mechanisms of two FA complex role models.One Is a multi-factor model:In this model,proteins interact with the protein binding site to form a FA compound,and then play a role.another is the cascade model:only after a certain FA protein bonding with DNA site,another free FA protein can bind to,progressing in turn,playing a regulatory role.FANCF protein is important to FA core compound,which play a role of engagement.many informations testify that abnormity of FA gene guide to chromosome breakage,damage of DNA,FANCF produces a marked effect through FA way,the damage of FA protein has been confirmed to cause ovarian cancer,oral cancer,lung cancer and cervical cancer.But the study on AML and MDS is rare.AML,MDS are no-expugnable problems in today's word of medical science,which is a serious treatment to human's healthy and life.The current incidence rates are rising trend year by year,and the existing chemical and bone marrow transplantation in the treatment of the disease can't reach complete healing,and treatment going together with certain body injury,in recent years,targeting tumor blood immune therapy research become a hot spot.Determine the specific tumor cell surface marker and expression of abnormal protein,design monoclonal antibody targeting tumor cells directly,or develop cancer vaccines,clean up tumor cells in the premise of no-damage to normal tissue,arriving the purpose of a long-term radical.We hope to establish base for gene therapy through comfirming the dependability of FANCF and AML,MDS. Approach for the relationship of pathogenesy with prognosis of AML and MDS.Provid new strategy for therapy.Materials and method 1.the study objects84 AML patients of first hospital of CMU OPD and Wd science september 2006 to June 2007,containing 46 men,48 female.M-F 1.2:1,MS-age is 54,preliminary diagnosis 58,complete remission 26;patients of MDS are 20,low-risk group has 9 specimens(RA 5,RAS 4),high-risk group has 11 specimens(RAEB 7,RAEB-T 4), MS-age is 69,M-F 1:1,control group has 20 speciments,bone marrow hints anaemia(megaloblastic anemia,renal anemia,hypoferric anemia)and thrombocytolytic purpura.,diagnosis are consistent with FAB and final diagnosis.2,bone marrow is extracted mono-nucleus cell by Flcoll density gradient centrifugation.3,detect FANCF protein by histochemical stain.4,detect FANCF protein by Western blot.5,Statistics methodAnalyze WITH spss 12.0,rank sum test for measurement data,X -test for enumeration data,Descriptive analyses adopt median.Results1,The expression of FANCF protein in AMLhistochemical stain show that The expression of FANCF protein in preliminary diagnosis group is 5.0%,CR group is 86.5%,control group is 75%;Western blot show that The expression of FANCF protein in preliminary diagnosis group is 27.58%,CR group is 92.21%,control group is 90.0%;is significantly lower in preliminary diagnosis than in control group(p＜0.05),comparing preliminary diagnosis group with control group,CR group has statistically significant(p＜0.05);there is obvious different between preliminary diagnosis group and CR group.Comparing CR group with control group has no statistically significant(p＞0.05).2,The expressions of FANCF protein in MDShistochemical stain show that express rate of low-danger group is 4.5%,high -danger is 1.0%,control group is 75.0%;western blot show that low-danger is 0.0%, high-danger is 0.0%,control group is 90.0%,comparison has statistically significant (p＜0.05).ConclutionThrough this experiment we can demonstrate that FANCF protein resides in normal human,and makes effect,first visit group of AML and MDS show obviously low-expression of FANCF protein than control group,which elucidates that FANCF protein is concerned with onset of AML and MDS.The difference between first visit AML and CR group indicatings that FANCF protein is concerned with prognosis of leukemia. |
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