| Along with the wide application antimicrobial agents,staphylococci have become an important source of nosocomial infection.The resistance of methicillin resistant staphylococcus epidermidis(MRSE) has increased even more than the resistance rate of MRSA.And caused more and more difficulities for clinical therapy.We evaluated the two methods for detection of MRSE,and analysis the resistance of staphylococcus epidemidis isolated from 20 hospitals in Anhui from January to December in 2005.Based on the study of molecular biological mechanism and molecular epidemiology,test the gene of MRSE.The results of present study may contribute to clinical therapy and molecular epidemiology of MRSE.Objective:Investigating the resistant of MRSE,evaluating the two methods for detetion of MRSE.Investigating the homology in MRSE isolates.Methods:MRSE were detected by cefoxitin disk diffusion,as well as PCR amplification for the mecA gene,respectively.128 strains of S.epidermidis isolates were tested for minimus inhibitory concentration(MIC) to 17 kinds of antimicrobial agents with agar dilution method.Taking enterobacterial repetitive integenic consensus(ERIC) as primer binding site,analysis the homology in MRSE by PCR.Results:The rates of MRSE detected by cefoxitin disk diffusion as well as PCR amplification for the mecA gene were79.69%and 85.16%,respectively,and the result of two groups had no difference(P＞0.05).Cefoxitin disk diffusion method of the specificity and sensitivity were higher enough to replace mecA PCR in clinical work.Except penicillin-G,resistance rate of MRSE to erythromycin and clindamycin were high too.And all strains were sensitive to vancomycin.Some isolates of MRSE have same fingerprint of genotype.Conclusions:Cefoxitin disk diffusion method can be used reliably in detecting and conforming MRSE.Resistance in MRSE was getting more and more serious,and some MRSE strains had clonal dissemination.So detection and surveillance of them should be emphasized. |
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