The Cell Bioeffect Study Of Several Au-based Nanoparticles

With the fast development of nanotechnology, the bioeffects of the nanomaterials have gradually become a new focus in the research fields flowing the research and exploitation of nanomaterials. Recently, gold nanoparticles as one of the important nanomaterials display several features of straightforward synthesis and easily modification, which make them be widely applied in biomedicine. However, the bioeffects of gold nanoparticles have not been systematic studied. This thesis is aimed at the cell bioeffects of Au-based nanoparticles. A series of studies on cell bioeffects of Au-based nanoparticles have been operated. The main results are as follows:(1) Establishing the methods of mouse embryo fibroblasts culture and subculture stability. Then the cultured fibroblasts were used to detect the cytotoxic reactions of near-infrared sensitive Au-Au₂S Nanoparticles. Discussion the possibility of toxicology testing on mouse embryonic fibroblasts cultured in vitro. To explore an economic, stable and rapid cytotoxicity evaluation methodologies of foreign compounds.(2) Based on Au, we synthesized the near-infrared-sensitive drug delivery vector Au-Au₂S nanoparticles ,to further investtigate biological safety of NIR sensitive cisplatin-loaded Au-Au₂S nanoparticles, four different cell lines were used to investage the acute cytotoxicity, cell apoptosis, and the long term potential carcinogenicity in vitro. our in vitro short and long-term data provided preliminary evidence suggesting the cisplatin-loaded Au-Au₂S nanoparticles are non-toxic below the maxium recommended dosage.(3) The toxicity of three diferent shape and different size gold nanoparticles to CHL cells was investigated by MTT analysis, flow cytometry technology and Hoechst 33258-based analysis. Results indicated that the cytotoxicity on CHL cells were concentration-dependent, and the following order was observed: rod>cauliflour>cube>sphere. No significant difference was observed among three different size. ICP / MS and TEM were used to study the absorption of nanoparticles on three different cells (Hela, NIH/3T3, CHL) and, in parallel observation of nanoparticles distribution in cells, The results showed that 30% nanoparticles were uptaken by various cell lines. TEM results show that the different shape of nanoparticles can enter through the cell membrane and randomly distributed in the cytoplasm.