Study On The Main Fungi From Rice Qu Of Yongxin Fermentated Turnip By The RDNA-ITS And Nutritive Ingredients Analysis

The flavour of Yongxin fermentated turnip was delicious produced by traditional Process for long.In this paper,we studied on the main microorganism of rice Qu of Yongxin fermentated turnip and the main nutritive ingredients of Yongxin fermentated turnip firstly.The main contents are illustrated as following;1.Identification of the main fungi from rice Qu of Y Yongxin fermentated turnip based on the rDNA internal transcribed spacer.Three fungi strains were isolated from rice Qu starter by potato-agar medium.The three main fungi strains were identified as Aspergillus oryzae strain FG41,Aspergillus oryzae strain SRRC 2103 and Aspergillus niger strain UWFP 515 by sequencing and sequence contrast(DNA extracted,then amplification of the whole ITS sequence including ITS1 and ITS2 by primer ITS1 and ITS4 by use of ITS rDNA).2.Studies on optimizing the conditions of producing enzymes by the main fungi from rice Qu of Yongxin fermentated turnip.Response surface methodology(RSM)based on the Box-Behnken design of experiment was used to optimize Solid-state Fermentation Producing enzymes.The factors selected for investigation were inoculation content fermentational temperature and fermentational days,By analyzing the response surface plots and the quadratic regression equations,the optimai conditions for obtaining the activity of proteinase to 524 U/g are 6.6×10~5 cfu/g,34℃and 7d,respectively.the optimal conditions for obtaining glucoamylase to 1476 U/g are 8.8×10~5 cfu/g,34℃and 7d,respectively.The optimal conditions for obtaining a-amylase to 877 U/g are 9.5×10~5 cfu/g,33℃and 7d,respectively.The optimal conditions for obtaining the activity of three enzymes to 520 U/g,1472 U/g,871 U/g are 9.4×10~5 cfu/g,34℃and 7d,respectively.The experimental data under various conditions have validated the theoretical values. Yongxin fermentated turnip were determined and the Nutritive ingredients were evaluated.The results showed that water,ash,total sugar,total acids,crude fat, crude fibre,protein,salt was 34,21.3,21.6,2.16,3.28,2.90,3.53 and 10.39 g/100 g,respectively; The content of 18 kinds total amino acids was 2.5 g/100 g and it included 8 kinds of essential amino acids,the ore element was abundant expect the content of Se was low,The content of K,Na,Ca,P,Mg,Fe,Zn was 264.1, 4691.5,192.0,45.91,51.03,5.118 and 1.514 mg/100g,respectively.