Studies On The Anticoagulation Active Fraction Of Tremella Aurantialba Mycelium

The purpose of paper is to screen and extract the anticoagulationactive fraction from Tremella aurantialba, study their efficacy in vitro andin vivo, and also study premilinarily the anticoagulation mechanism.By applying the methods of chemistry and pharmcodynamic thestudy on screening the anticoagulation active fraction have beencompleted. The total coumarin component ultimately were proved to bethe best active anticoagulation active fraction. Conditions for theextraction were studied by orthogonal design and UV-spectralmethod, guided by the content of coumarin.The best extraction processwas determinded to be three refluxs whth six times of 70% alcohol forone hour.The effective constituents were accumulated by the using the contentof coumarin as index. Firstly, D101 macropore absorbed resin waschoosed as the optimal technique for isolation,and the concentration ofsample was 0.05g/ml,the flowing of speed of absorption and unabsorptionwere 1.0ml/min.Secondly, parameters in the concentration and volume ofthe elution were investigated mainly. The technique of preparation wasestablished as following:eluting with water till colourless, eluting with50% alcohol(200ml).The contents of the total coumarin of Tremellaaurantialba in large experiments were 34.84%.The active fraction would contribute to the experiment of pharmcodynamics later.By using the content of coumarin as index and inspectings ofprecision, stability, repeat and the average recovery. The method ofdetermination of content for the total coumarin of Tremella aurantialbawas established. By doing the anticoagulation experiment, the totalcoumarin of Tremella aurantialba were again proved to be the bestanticoagulation active parts.The conclusion would supply the basis forproduction and studying later.