| QDPTP is an compound extractive of several kinds of the traditional Chinese medicine including Astragalus mongholicus, salvia miltiorrhiza, szechwan lovage rhizome, Carthamus tinctorius and so forth. It has been proved that QDPTP protect heart damaged by ischemia / reperfusion (I/R). And the aims of this study were to confirm the protect effect of QDPTP in experimental cerebral ischemia / reperfusion model and further clarify the mechanisms of actions by using different experimental methods. The main results are as follows:Part I QDPTP decreased cerebral injury induced by focal cerebral ischemia / reperfusion in rats1. The effects of QDPTP on the neurological deficits scores after I/R in ratsFocal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) for 2 hours and followed reperfusion for 4 hours. After that, we estimated deficit scores grading on a scale of 0-5. QDPTP (450, 900, 1800mg/kg) and Lig (33mg/kg) were found significantly decreasing neurological deficit scores after I/R (P<0.01).2. The effects of QDPTP on cerebral infarction size after I/R in ratsThe cerebral infarction size was measured by TTC staining technique after I/R. QDPTP (450, 900, 1800mg/kg) and Lig (33mg/kg) evidently decreased the cerebral infarction size after I/R (P<0.01).3. The effects of QDPTP on cerebral water contents and cerebral index after I/R in ratsWe measured the cerebral water contents with wet/dry weighing method and surveryed cerebral index after I/R in rats. The results showed that QDPTP (450, 900, 1800mg/kg) groups and Lig (33mg/kg) cut down both cerebral water contents (P<0.01 or P<0.05) and cerebral index.Part II Mechanism of protection of QDPTP from cerebral ischemia/ reperfusion in rats1. The effects of QDPTP on oxidative damage and inflammatory reaction after global cerebral I/R in ratsA. The effects of QDPTP on cerebral SOD activities and MDA contents after global cerebral I/R in ratsThe model of global ischemia / reperfusion was established by improved Pulsinelli's method for 20 minutes'occlusion and 24 hours'reperfusion. The activities of SOD and the contents of MDA in cerebral tissue after I/R were tested respectively. Comparing with NS in model group, QDPTP (450, 900mg/kg) lowered the contents of MDA (P<0.01) and reserved the activities of SOD(P<0.01). These effects of QDPTP (450, 900mg/kg) were comparable with Lig (33mg/kg).②The effects of QDPTP on IL1-βcontents of cerebral tissue and blood serum after global cerebral I/R in ratsIn the model group the IL1-βcontents of cerebral tissue and blood serum messured by ELISA were markedly increased after global cerebral I/R in rats. Comparing with the model group, the IL1-βcontents of cerebral tissue and blood serum decreased significantly in QDPTP (900mg/kg) group (P<0.01) and Lig (33mg/kg) (P<0.05) .2. The effects of QDPTP on cerebral blood flow and artery blood pressure in anesthetized ratsThe regional cerebral blood flow (rCBF) in cerebral pia mater was examined by Laser Doppler Flowmeter and the artery blood pressure (including mean systolic pressure, mean diastolic pressure and mean blood pressure) were monitored by the electrophysiological polygraph before and after drug administration. Comparing with the solvent, QDPTP (450, 900, 1800mg/kg), Lig (33mg/kg), Nim (20mg/kg) and DSDW (60mg/kg) expanded rCBF (P<0.01 or P<0.05) and simultaneously decreased the artery blood pressure (P<0.01 or P<0.05).Conclusion1. QDPTP had protective effects on the brain damaged by acute local and global cerebral ischemia/reperfusion in rats.2. QDPTP improved neurological function and decreased the infarction size and cerebral edema induced by acute local cerebral ischemia /reperfusion in rats.3. The mechanism of anti-cerebral injury of QDPTP in cerebral ischemia / reperfusion model may be related to the inhibition of the damages caused by oxidative free radicals, the extenuation of inflammatory reaction mediated by cytokines, the extension of cerebral vessels and the increase of cerebral blood flow.
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