Research Of Conservancy And Perfusion Of Extracorporeal Liver

Backgroud: The liver transplant currently is a effect way to cure liver dieases in last phase, there is relationship between cold conservancy re-perfusion and dyfunction ,disorder of primary transplantation liver after operations. How to raise the liver protection quantity and lower the harm of blood- infuse and improve the liver transplantation is important topic that current scholar concernCurrently that realm research in the usage infuses to flow the method circularly continuously, regard the living creature artificial liver as principle, addition blood or blood plasmas infuse to constitute outside combination an artificial liver assistance equip, become research most of, the most hopeful artificial liver system. Hope to acquire the better path of the liver conservancy through this experiment, create a body of nature inside circulation environment, prolong liver conservancy time Do some foundation researches for the clinical application that the liver infuses to flow the system Objective: To study the founction of perfusion in liver Conservancy , through build up the model of perfusion extracorporeal live.Methods: Porcine livers were harvested from common health dogs, performed by dual (portal vein and hepatic artery) vessel perfusion and oxygenating blood together. Observed the case of pathology variety , recorded the bio-chemical index sign of lack blood time , gall secretes , perfusion pressure, guts analysises and ammonia (ALT,AST).Results: Along with perfusion time to lengthen, the sour alkalinity starts go sour gradually, the liver metabolism lactic acid starts increase, the oxygen presses to present to descend the trend separately, the CO2 presses separately to go up gradually, the ammonia (AST, ALT) to go up gradually in the liquid, the bilirubin go up, the total protein and ALB contents is normal. About 24 -36 hours all liver appears swell plaque in dark red , gall go down , presses rise quickly. At the end of ECLP, the liver cell to swell, to different degree die and to collapse.Conclusion: Pass to experiment above, complete the big animal liver to leave the body to infuse to note, prove in the 24-36 hs the liver leave the body infuses to note can the basic maintenance liver of physiology and bio-chemical function.