Study On Novel Analytical Method Of Ciprofloxacin Hydrochloride

In the antibiotics family, quinolones antibiotics are the only kind synthesis antibacterial drugs which can compare beauty with lactam kind antibiotics. They have the fast sterilization effect for most sensitive bacteria. Ciprofloxacin are widely applied to the clinical medicine as one of the most successful third generation quinolones drugs. Due to many characteristics such as broad spectrum, efficient, low toxicity and complete oral absorption, wide distribution, hard to cause drug-resistance, Ciprofloxacin has been one of the best anti-infective drugs at present.In the present study, three new analytical methods for the determination of ciprofloxacin hydrochloride were developed. These methods are not only used to evalue the effective component which is as one of the most imporment pharmacodynamics indexes, but applicable in the analysis of pharmaceutical residues like antibodies in environment samples as well as in foods. The major results of thepresent study are listed as below:1. A fluorescence quenching method for the determination of ciprofloxacin hydrochloride by fluorescence resonance energy transfer between acridine orange and rhodamine BThe fluorescence resonance energy transfer between acridine orange and rhodamine B has been studied. It was found that the effective energy transfer occurred between acridine orange and rhodamine B in the sodium dodecyl sulfate atλex /λem = 470 / 576 nm and Britton-Robinson buffer(pH = 7.0). The fluorescence intensity of rhodamine B which had increased sharply in the energy transfer system was regularly diminished by ciprofloxacin hydrochloride, and the quenching fluorescence intensity showed a good linear relationship with the concentration of ciprofloxacin hydrochloride in the range of 0.02 to 0.44μg/mL. The equation of linear regression was△F = 378.0c(μg/mL) + 11.14(correlation coefficient r = 0.9966), the detection limit was found to be 0.013μg/mL. The method was applied to the determination of ciprofloxacin in ciprofloxacin hydrochloride eye drops. It was validated applying the standard addition methodology and using HPLC as a reference method.2. Fading spectrophotometric method for determination of Ciprofloxacin Hydrochloride with Congo RedIn weak acidic of Britton-Robinson buffer medium, ciprofloxacin hydrochloride reacts with Congo Red to form an ion-association complex,and the absorption of Congo Red decreases obviously.The maximum fading wavelength at 496nm.Based on this reaction, a new fading spectrophotometric method was developed for determination of ciprofloxacin hydrochloride. The equation of linear regression was△A=0.03257c(mg/L)-0.0245 (correlation coefficient r = 0.9980) in the range of ciprofloxacin hydrochloride concentration in 0.8—8.0mg/L, the detection limit was found to be 0.3376 mg/L.The method was applied to the determination of ciprofloxacin drugs with good results.3. Complexation characteristics of ciprofloxacin with Zn(Ⅱ)and determination of ciprofloxacinThe complexation characteristics of ciprofloxacin(CPFX) with Zn(Ⅱ)has been studied. The complexing ratio of the complex has been determined to be 2:1 using molar ratio method,and the degree of dissociation and stability constant were calculated. Based on this complex reaction, a simple, rapid and sensitive new method for the UV determination of CPFX was proposed and the reaction conditions were investigated in detail. The method was applied to the determination of ciprofloxacin drugs with good results.