Analysis Of Optical Clearing Of Blood With Mie Theory

Optical clearing is a technique to increase the light penetration depth in the tissue by the application of clearing agent to reduce light scattering and match the refractive index between the scatterer and the environment. The experiment reported that apart from the index matching, the valume also changes in the clearing progress and affects the results of optical clearing. To study the influce of both valume and index matching on the optical clearing, as an example, the author analysed the optical scattered by the human blood with Mie theory,and got the condition to achieve the best penetration.Based on the the light characteristics of RBC,a ball model was adopted as a scatterer approximately,and Mie theory and Fraunhofer approximation were used to study the aggregation and the relative refractive index of red blood cells to blood plasma, on the optical clearing of blood, The results showed that the transmission light enhances as the aggregation increases, and to maximate the forward scattering light, a smaller relative refractive index is needed for a lower level of aggregation. This implied that a best optical clearing can be achieved in the optical dynamic progress when theconditions mentioned above are met.