The Level Of CGRP In The Asthma Lung In Rats

Objective To explore the role of sensory neuropeptide calcitonin gene-related peptide (CGRP) and the antagonist CGRP (8-37) in the pathogenesis of the rat model for asthma. Methods SD rat (mail) were divided into asthma group (A, n=8), CGRP(8-37) interfering group (B, n=8) and normal control group (C, n=8). The CGRP level of Right lobes from rats exposed to 1% ovalbumin (OVA) for seven episodes (30 min each day) or filtered air (Group B are Pre-treated with the antagonist CGRP (8-37) before peritoneal injection OVA), were detect with histochemistry. The concentration of CGRP in blood and in BALF was assayed by ELISA . Results: 1. The lymphocyte and eosinocyte number of BALF in A group were significantly higher than those in B and C (P<0.01). 2. CGRP-positive cells in A group were significantly higher than those in B and C (P<0.01). 3. The gray scale in A group were higher than those in C and B (P<0.05). 4. The concentration of CGRP in blood and BALF were differential between A and C (P<0.05). Conclusions: The data suggested that CGRP may influence the exacerbation of symptoms associated with asthma, and CGRP(8-37) can release the airway inflammation and hyperreactivity.