| ObjectivesThis research is to establish the model of rats of chronic renal damage wasinduced by adenine.To observe the effect of different dose soy isoflavone onnutritional status and lipometabolism in rats of renal damage.Whether it havesignificant differences between different does soy isoflavone.To evaluate soyisoflavone can improve lipometabolism and nutritional status. How much SIF doseeffective were intaked in rats model of chronic renal damage,so as to provide anexperimental basis in for guiding nutrition nursing.Methods1.Division of groups: To select Wistar rats (n=30)were divided randomly intothree groups,including basis diet group(to intake partes aequales arachis oil), low SIFgroup(SIF dosage is 6mg/kg.d)and high SIF group(SIF dosage is 12mg/kg),Therewere 9 rats each group.2.Preparation of chronic renal damage model:The rats were intaked 2% adeninesolution(200mg/kg) every day,It were two weeks,Behind two weeks, 2% adeninesolution dose were changed 100mg/kg every other day for one week,All time is 3weeks.The model of chronic renal damage was established by this method. Blood biochemical indicator and pathological sections confirmed the establishment of themodel of chronic renal damage is successful.3.Observation of general condition of rats model:Diet,activities,color and luster ofhair, body weight were observed during the experiment.4.Measurement of biochemical indicator:The levels of TG,CHOL,HDL,LDL,TP,ALB,BU,Scr and UPE were measured separately at different times of theexperiment,including before (T1) and after chronic renal damage on 42th day(T42).5.Pathological sections investigation of renal tissue: The pathological sectionchanges of the renal tissue were analyzed after establishment of the model of chronicrenal damage.6.Data analyze: The collected data was analyzed with the statistical package SPSS11.5.Results1.General status:With the adenine solution intake,the amount of drinking waterand urine volume obviously increased,but the food-intake decreased.The mental stateand physical activities of rats were obviously reduced.There were hyperproteinuriaafter established model. The mental state and physical activities of SIF controlgroups were obviously improved than that of basis diet group, The hair becomessmooth and glossy after treatment for two weeks.By the end of the experiment (the42th day),The weight of the three groups increased than that before theexperiment,but showed no statistical significance(P＞0.05).During theexperiment,one rat died in basis diet group on the third day, another rat died in highSIF group on 15th day.2.Renal function parameters: Before the experiment, Three groups have notstatistical significance on BU,Scr and UPE (P＞0.05). BU,Scr and UPE of two SIFcontrol groups were decreased on the 42th days,But basis diet group has not obviously changes. By the end of the experiment (the 42th day),The concentration ofBu,Scr and UPE have statistical significance compared with basis diet group,thelow SIF group and the high SIF group. F value were 8.239, 5.218 and 11.051respectively. P value were 0.002, 0.014 and 0.000 respectively. Compared with basisdiet group and two SIF control groups,It has statistically significant difference (P＜0.05).But compared with two control groups has no statistically significant(P＞0.05).3.Blood-lipoids parameters: While all the rats of renal damage were randomlydivided into three groups, Three groups have not statistical significance onTG,CHOL,LDL and HDL (P＞0.05).After the 42th day, All above parameters keepsource level in basis diet group. That have obvious changes in SIF controlgroups,Level of TG,CHOL,LDL have decreased, HDL has advanced. By the endof the experiment (the 42th day),the concentration of CHOL,TG,HDL,LDL havestatistical significance compared with basis diet group,the low SIF group and thehigh SIF group. F value were 27.405, 24.617, 21.261 and 22.597 1 respectively. ALLP value were 0.000. Compared with basis diet group and two SIF control groups,Ithas statistically significant difference (P＜0.05).But compared with two controlgroups has no statistically significant(P＞0.05).4.The result of TP and ALB: By the end of the experiment (the 42th day), theconcentration of TP in three groups(basis diet group,low SIF group,high SIF group)were (73.55±3.54) g/L,(78.50±3.63) g/L,(79.88±3.98) g/L, F=6.462,P=0.006respectively, the concentration of ALB in three groups were (27.83±1.60) g/L,(32.15±0.97) g/L,(32.47±1.17) g/L F=33.936,P=0.000.The concentration of TP andALB of two SIF control groups were higher than the basis diet group.It has statisticalsignificance(P＜0.05). But compared with two control groups has no statisticallysignificant(P＞0.05).5.The results of pathology in renal tissue:After established model,Number of the kidney glomerulus reduce, A great quantity of necrotic renal tubular wereobturated.there are a lot of Inflammatory cell infiltration and obvious interstitialfibrosis.Conclusion1. SIF can improve the lipometabolic disturbance and protect renal function in ratsof renal damage.2. Low dose SIF can improve the nutritional status in rats of renal damage.
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