| Objective: To assess whether increased Notchl signaling andtranscription factor c-Fos expression during cervical cancer progressionfrom non-dysplastic (inflammatory or reactive) cervical tissues, preinvasive(comprising of LSIL,HSIL), and invasive squamous cell carcinoma,andinvesgate the potential relationship of them. Methods: A total of 110cervical samples, which included 15 non-dysplastic cervical tissue, 30preinvasive lesions and 65 squamous cell carcinomas, were analyzed for theexpression of Notchl,c-Fos, by immunohistochemistry. The expressionwas classified as mild (+), moderate (++) and intense (+++) based on theintensity of immunostaining. Statistical analysis was carried out using SPSSversion 11.5. Analyses showing a confidence interval of 95％(p＜0.05) wereconsidered significant. Results: In non-dysplastic Cervical samples, Notchlshowed 36.36％cellular positivity of cytoplasmic expression, while c-Fosshowed 20％with nuclear localization. Then, it is 90％of Notchl and 20％of c-Fos in pre-neoplastic lesions, and 96.93％and 95.38％respectively ininvasive carcinoma. During cervical cancer progression from normalepithelium to invasive carcinoma, Notchl and c-Fos expression increased drastically (P＜0.05 ). It was seen that 59％of the cases that were Notchlpositive and c-Fos positive. These results therefore reveal coexpressionintracellularly of the relevant components of Notch and c-Fos. In invasivecarcinomas, the significant increase was observed in the number of positivecases and elevated levels of c-Fos, it also associate with and the ability totumor progression and infiltration. The results also reveal the features ofdisregulated Notch signaling during tumor progression, and associated withpatients' age,progression of cervical cancer to advanced stages. Conclusion:Our results suggest that there probably be a co-activation of c-Fos andNotchl signaling pathways at the cellular level in the majority of humancervical cancer.
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