| Objective: The study aimed at identifying by PCR that if distinction exist in Trichophyton rubrum strains isolated from different patients in one family. To analyze the source of strains caused dermatophytosis in one family is from family internal members(home onset of infection) or from other ways besides family internal members(infection outside home).Methods: The scales scraped from patients with dermatophytosis in same family were inoculated on SDA. On physiological and morphological characteristics, the isolates were identified to species. If strains from different patients shared the same species, then used the RAPD and specific amplification of tandem repeat subelements (TRSS) in strain typing and compared the products.Results: In this study, all of these strains isolated from 16 patients in 8 families (2 patients each family) were identified as Trichophyton rubrum by morphologic method and others. In 4 families, the distinction between strains were identified by RAPD with 4 arbitrary primers. In 5 families it was found that the isolates from one family were different on the types of finger print produced by amplifying TRS-1 specifically, which produced the most genotype. And 2 families were different by amplifying TRS-2. Aggregately analysis various kinds of typing methods, they shown the difference of the strains came from 6 families (75%), the strains isolated from the others (only 2 families) couldn't be identified difference.Conclusions: 1. Finger print produced by specific amplifying TRS-1 has more strain characteristic than RAPD for distinguishing strains between T.rubrum spp, and it is fit for strain typing in T.rubrum. 2. Strains contrasted each other can be identified different just by any strain typing method. 3. Combination of the two methods RAPD and amplification of TRSS will display the distinction in T.rubrum strains to grate degree. Among 8 families, the contrast in Trichophyton rubrum Strains from different patients of one family were identified in 6 families(75%). |
