Effect Of The Extractive Of Glycyrrhizic,Gymnemasylvestre Schult On The Streptococcus Mutans

Dental caries is the common disease and still prevalent among the mankind's oral cavity,the World Health Organization has already listed it as one of three the mankind noncontagious diseases . Streptococcus mutans is one of the dominating and resident germs of oral, also is the dominating germ factor of the occurrence of caries. the Fluoride is the matter already know comparatively effective defending against caries, its function to Streptococcus mutans especially to the it's some enzyme effects , reduce the occurrence of caries by disturbing the bacterium's regularity growth. The recent years researches have discovered some natural medicine compositions,such as Nidus vespae,Galla chinesis...etc., also can inhibit the growth and yield acid of Streptococcus mutans ,and have significant anticaries effects in vitro and in biofilm model. Glycyrrhizic acid and gymnemasylvestre schult are always think to have the function in the Chinese herbal medicine of antimicrobial and antiphlogistic.but have not been applied in the Dental caries medical treatment currently.Therefore, the purpose of this study is to investigate their anticaries effect,to evaluate the anticaries efficacy of the active component in vitro and in biofilm model.Streptococcus mutans has been chosen as the experimental bacteria.Two experiments were carried out.It is evaluated the inhibiting ability of the natural medicines on the growth of the bacteria by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) to the streptococcus mutans and comparing the cariostatic effect among glycyrrhizic acid,gymnemasylvestre schult and sodium fluoride through growth inhibiting experiment in votio and in biofilm model.We mensurate the absorbency of bacteria growth,record the pH,compare the ability of anticaries among the three agents.The results shows that, the MIC of glycyrrhizic acid and gymnemasylvestre schult are 0.625%,2.5%; the MBC of glycyrrhizic acid is 10%, gymnemasylvestre schult is not the able to sterilize streptococcus mutans.The effect of glycyrrhizic acid and gymnemasylvestre schult on the Streptococcus mutans is influenced by the concentration of themselves. When the concentration of glycyrrhizic acid≤0.25%, gymnemasylvestre schult≤1.25%,the effect of them on the regularity growth and yield acid of this bacteria is small,when the concentration of gymnemasylvestre schult is 2.5%,the effect of it on the yield acid of this bacteria is small ,too.When the concentration of glycyrrhizic acid≥0.5%, gymnemasylvestre schult≥2.5%,the effect of them on the regularity growth of this bacteria is great in the first 32h,but it is little after the 32h.When the concentration of glycyrrhizic acid is 0.5% and l%,and gymnemasylvestre schult is 5% and 10%,the effect of them on the yield acid of this bacteria is great in the first 32h,but it is little after the 32h. When the concentration of glycyrrhizic acid is 2.5%,the yield acid of the streptococcus mutans is almost stoped.Based on these flndigs, glycyrrhizic acid and gymnemasylvestre schult can inhibit the growth of the streptococcus mutans,and disturb yield acid of this bacteria, especially in the first 32h after the accession of the natural medicine.Though the concentration of the natural medicine is higher,the natural medicine have application vice- respond little,bad function to the body is small, not easy induce the anti-medicine germ and the secondary infections, so also have good foregrounds.