Primary Hepatic Carcinoma: Blood Supply By Computed Tomography, Serum Anti-p53 Antibody And Serum Vasscular Endothelial Growth Factor

Background: p53 gene is a kind of anti-oncogene which is closely related to human tumor. Following mutation of p53 gene is the expression of aberrant p53 protein which has a rather prolonged half life and increased stability to induce autoimmunoreaction with the production of anti-p53 antibodies. Anti-p53 antibodies can be used as a specific marker of tumor to indirectly reflect mutation of p53 gene and aggregation of intracellular p53 protein, which has certain value in early diagnosis, prognostic estimation, and screening in high risk populations.Primary hepatic carcinoma (PHC) belongs to solid tumors with abundant vascularity. Blood supply of PHC is closely related to growth, infliltration and metastasis of this malignant tumor, and can be well demonstrated by computer tomography (CT). As for the mechanisms of vascular formation, vascular endothelial growth factor (VEGF) is one of the most important growth factors and affects the vascular endothelium directly. VEGF can specifically increase the dividing, proliferation and movement of endotheliocyte, as well as the permeability of tumor vessels, making it a crucial angiogenic factor in research.Mutation of the p53 gene facilitates the formation of vessels. Until now, reports are very few as to the correlation between blood supply of PHC and serum anti-p53 antibodies, as well as serum VEGF.Aim: To investigate the correlation between blood supply of PHC and serum anti-p53 antibodies, and between that and serum VEGF, as well as the correlation between serum anti-p53 antibodies and VEGF.Method: Firstly, the enzyme-linked immunosorbent assay (ELISA) was used to quantitatively analyze serum anti-p53 antibodies and VEGF in PHC with different blood supply. Then levels of anti-p53 antibodies from patients of PHC with different blood supply were investigated to see if there is difference (univariate analysis). Also, levels of serum VEGF from these patients were examined for difference (univariate analysis). Finally, the interrelationship between serum anti-p53 antibodies and VEGF was studied.Results: Of the 80 cases of PHC, univariate analysis demonstrated that there was significant difference between levels of serum anti-p53 antibodies (P＜0.05) and between levels of serum VEGF (P＜0.05) in PHC with different blood supply. In some types, serum anti-p53 antibodies were positively correlated to serum VEGF.Conclusion: There is conspicuous difference between levels of serum anti-p53 antibodies and between levels of VEGF in PHC with different blood supply. Additionally, there is a positive correlation between serum anti-p53 antibodies and VEGF.