| Tetramine is a kind of azacyclo-rodenticide with a small toxic dosage. Tetramine is a colorless, tasteless and stable substance. Poisoning which caused by taking it by mistake is very common as well as in suicide or criminal cases. It is of a high death rate. At present, there are many researches on the clinical manifestation and the symptomatic treatment of people poisoned by tetramine, while there are few the foundation research references of its toxic mechanism. Therefore, its toxic mechanism hasn't been elucidated. This research aimed at showing the pathological change of poisoned rat and the relationship between the tetramine quantity and poisoning time, providing science basis for revealing its toxic mechanism, for the clinical diagnosis and treatment of people poisoned by tetramine, providing reference for medicolegal expertise of cases caused by tetramine.In order to determine the tetramine concentration in blood and tissues, after inspecting some extracting solvent and optimizing chromatographic condition. The analytical method for tetramine in biomaterial was established in this study. Tetramine was extracted by benzene and determined by GC-TSD which will describe as follow. A VARIAN CP 3800 gas chromatography equipped with a nitrogen/phosphorus detector (TSD) was introduced in this paper to determinate tetramine. Compounds were separated on a CP-Sil5CB fused silica capillary column coated with dimethyl siloxane thickness 0.25μm 30m×0.25mm i.d. The column temperature program was set as follows: 100℃(?) 210℃(2min) (?)280℃(3min). Nitrogen gas was used as the carrier gas, at a flow rate of 1mL/min.We used the retention time of tetramine standard for qualitation, external reference method for quantitation.In this study, we used healthy SD-rats as experimental animal, divided them to five and a control group at random. The rats in the experimental groups were used for poisoning experiment. Some of them were poisoned with different dosage then killed at the same time. Others were poisoned with the same dosage then killed at different time. The control group was given physiologic saline as blank. We collected the blood, heart, liver and brain of every rat, determined tetramine in these samples of experimental groups, used the solution consisted of paraformaldehyde and glutaraldehyde to fix tissues, applied light microscope and transmission electron microscope to observe pathological changes. In the end, we investigated the pathological change and the relationship between tetramine quantity and the poisoning time.The pathological change observed by the light microscope show that teteamine could injure the brainstem, heart and liver of poisoned rats without obvious sex differences. The degree of heart injury did have some thing to do with tetramine quantity and the poisoning time. While the degree of brainstem and liver injury did not have distinct dependablity relativity to tetramine quantity and the poisoning time.The pathological change observed by the transmission electron microscope show that there are dropsical cytochondria, reduction of ribosomes, normal gliacyte and neurofibra myelinolysis. The neurofibra myelinolysis caused by tetramine had a positive correlation to tetramine quantity and the poisoning time. The higher concentration of tetramine in brain tissue and longer poisoning time, would cause a worse neurofibra myelinolysis.In this study, we established the method for detecting tetramine in biomaterial, investigated the pathological change and the relationship between tetramine quantity and the poisoning time. The results could provide reference for further study on toxic mechanism of tetramine and offer valuable gist for the clinical diagnosis and treatment of people poisoned by tetramine, providing reference for medicolegal expertise of cases caused by tetamine. |
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