A Study On Associations Of Apolipoprotein E Polymorphisms With The CVS And Rebleeding After Spontaneous SAH

ObjectiveTo investigate the influence of the APOE gene polymorphisms (ε2,ε3,ε4) on cerebral vasospasm (CVS) and rebleeding of patients with spontaneous subarachnoid hemorrhage (SAH).Methods Eighty-four patients with spontaneous SAH admitted to the Neurosurgical Departments of the First Affiliated Hospital of Chongqing Medical University from February 2006 to November 2006 were selected in this study. Venous Blood samples and clinical data of the patients were collected. APOE gene polymorphisms were determined by PCR-RFLP. Associations of APOE polymorphisms with cerebral vasospasm and rebleeding after SAH were analysized byχ2 test, Uni- and multivariate logistic regression (SPSS verion 14.0).Results1.The relationship between genotypes and CVS. The distributions of APOE genotypes and alleles matched Hardy-Weinberg Law. In 84 patients, 13 of 17 (76.5%) patients with APOEε4 allels showed CVS, which was significantly different from those without APOEε4 allels (P=0.035). Uni- and multivariate logistic regression analyses demonstrated that ApoEε4 allels was a risk factor (Univariate logistic regression OR=2.058,95%CI 1.191-3.559,P=0.010. multivariate logistic regression OR=2.354,95%CI1.053-5.265,P=0.037).2.The relationship between genotypes and rebleeding. Though the ratio of rebleeding in either APOEε2 or APOEε24 carriers was higher than that of either non- APOEε2 or non- APOEε4 carriers repectively, no statistical significances were found (P=0.125 forε2, P=0.661 forε4). There was also no statistical difference in percentage of rebleeding between APOEε3 and non- APOEε3 carriers even though less cases of rebleeding were obversed in patients with APOEε3 (P=0.335).Conclusions Our findings suggeste that the patients with APOEε4 allele predispose to CVS after spontaneous SAH. The relationship between APOE polymorphisms and rebleeding after SAH is not verified and needs further investigation.