Expression And Correlation With Clinicopathologic Parameters Of Hugl-1 Gene In Human Lung Cancer

Objective The aim of this study was to assess the expression of protein products of Hugl-1 (human lethal-giant-lavae 1)in human lung cancer, to estimate their possible expression and to correlate the results of immunohistochemical analysis with various clinicopathologic parameters. Methods The expression of Hugl-1 gene was detected by immunohistochemical S-P method and Western-blot method in 50 cases of NSCLC tissues ,50 para-cancer tissues and 10 cases of lung benign lesion tissues as control. According to immunohistochemical results, the expressions of Hugl-1 mRNA were detected by reverse transcriptase polymerase chain reaction(RT-PCR) in corresponding samples. Results (1)The expression level of Hugl-1 gene was found to be significantly lower in NSCLC tissues than in para-cancer tissues and lung benign lesion tissues(P<0.05). (2)The expression level of Hugl-1 gene was closely related to PTNM stages and lymph node involvement in Lung cancer patients(P<0.05). Conclusions Maintenance of Hugl-1 gene may effect cell polarity,changes of epithelial polarity causes hyperplasia and reduction of Hugl-1 gene expression may be related to the pathogenesis and development of lung cancer.