Study Of Vascular Smooth Muscle Cell Calcification Induced By Hyperphosphate And Intervented By Atorvastatin

Objective: To study the effect and mechanism of atrovastatin on vascular smooth muscle cell calcification. Methods: Rat vascular smooth muscle cells (RVSMCs) were placed in various culture media, including normal phosphate medium, high phosphate medium, ZVAD.FMK medium and atorvastatin medium. In different time, we quantified the calcium deposition and apoptosis of RVSMCs. Results: Phosphate increased calcium deposition and apoptosis dramatically in a concentration-and time- dependent manner. Calcium deposition decreased dramatically after apoptosis being inhibited by ZVAD.FMK. Atrovastatin significantly suppressed calcium deposition and apoptosis in a concentration-dependent manner. Conclusions: Pi induces calcium deposition in RVSMCs.Atorvastatin inhibited Pi-induced RVSMCs calcification by preventing apoptosis.