The Research Of Ripe Porcine Sinoatrial Node Cell's Bionomics And The Initial Study Of Cell Autotransplantation

Objective:To obverse the histomorphology construction features of procine heart sinoatrial node by light and electron miscroscope.To cultivate the ripe procine sinoatrial node pacemaker cell and make the identification and autotransplanation. Methods:Locate procine sinoatrial node by electrophysiology mapping roughly. Draw the materials form node and sect serial sections, HE dyeing, and observe the form and tissue structure of procine sinoatrial node. Locate the node accurately and obverse the ultrastructrue by electron microscope.Draw the material from ripe procine sinatrial node and use them for cell primary culture.Observe the primary cultured cell,and record the electrophysiological characteristics by patch clamp.Autotrransplant the cultured cell into the procine heart and set up atrioventricular block animal model to identify the cultured cell's function. Results:Procine sinoatrial node situates the top of superior vena cava and right atrium juncture's terminal sulcus. Both P cell and T cell have characteristic form and distribution.According to the form and structrue's view and electrophysiology detection of the cultured cell, macrospindle cell consistents with the feature of sinoatrial node pacemaker cell.The atrioventricular block animal model after cell transplantation can confirm that the sinoatrial node pacemaker cell can work effectively. Conclusion:Procine sinoatrial node has differernt form and construction compared to other cardiac muscle tissue, and the cell's composition and function are coincidence with the sinoatrial node's function. The isolation and cultrue of ripe procine sinoatrial node pacemaker cell need a new and dependable cell cultured technique. The macrospindle cell is pacemaker cell. The primary cultured cell after transplantation can work effectively.