The Study Of Different Dose's Urokinase On Vascular Morphological Changes Of Acute DVT In Rats

Objective:To observe the effect of different dose's urokinase on morphological changes of vein wall after differenct periods of deep vein thrombosis(DVT)in rats.Methods:Firstly,96 sprague-dawley(SD)rats were randomly divided intoⅠ,Ⅱ,Ⅲ,Ⅳperiods on 1^st,3^rd,7^th,14th day following thrombosis respectively,there were 24 SD rats in every period.Then the 24 SD rats in each period were separated to 4 group.Low dose' s urokinase group(20,O00u/kg·d,n=6.),mod.dose' s urokinase group(40,000u/kg·d, n=6.),high dose' s urokinase group(80,000u/kg.d,n=6.),thrombosis group (0,000u/kg·d,n=6).In each group,to observe the remain quantity of endothelial cells and the expression areas of collagen by dyeing.Results: 1 SD rat in thrombosis group was died inⅡ,Ⅲperiod respectively.1 SD rat in mod.dose's urokinase group was died inⅢperiod before thrombolytic therapy.InⅠperiod,the quantity of endothelial cells and the expression areas of collagen in low,mod.,high dose's urokinase group have significant difference with thrombosis group respectively(P＜0.05).InⅡ,Ⅲperiod, the quantity of endothelial cells in high dose's urokinase group have significant difference with thrombosis group respectively(P＜0.05),low, mod.Dose's urokinase group haven't significant difference with thrombosis group respectively(P＞0.05);The expression areas of collagen in low,mod.,high dose's urokinase group have significant difference with thrombosis group reapectively(P＜0.05)。InⅣperiod,the quantity of endothelial cells in low,mod.,high dose's urokinase group haven't significant difference with thrombosis group respectively(P＞0.05);The expression areas of collagen in low,mod.,high dose's urokinase group have significant difference with thrombosis group respectively(P＜0.05).Conclusions:Different dose's urokinase have protective effect on the quantity of endothelial cells during 3 days after thrombosis;Only large dose's urokinase have protective effect on the quantity of endothelial cells during 3-14days after thrombosis; Different dose's urokinase haven't protective on the quantity of endothelial cells after 14 days following thrombosis,but they have inhibitary effect on the expression areas of collagen in every period,the effect of high dose's urokinase is better.