Effect Of Different Oxygen Conditions On Proliferation Activity Of Trophoblastic Cells

Objective To explore the effect of cell proliferation activity by establishing vitro culture model of trophoblastic cells in different oxygen conditions.And to explore whether there is protective effect on trophoblastic cells by exerting different concentration of anti-oxidant Mg²⁺.Methods To randomly select 20 cases of healthy women,who were 8 to 10 weeks of pregnancy and received induced abortion.All selected objects were excluded if there were factors effect on the results. The trophoblastic cells of primary culture were divided into the folio wing six groups to culture:normal control group,hypoxia group,hy poxia-reoxygenation group,hypoxia- reoxygenation with 0.6mmol/LM g²⁺group,hypoxia-reoxygenation with 1.2mmol/LMg²⁺group,hypoxiareoxygenation with 2.4mmol/LMg²⁺group.The MTT colorimetric ass ay was used to analyze the cellproliferation activity after these grou ps were cultured at 1,4,12,36,48,and 72 hours.Results 1.The proliferation activity of trophoblastic cells in hypoxia group had significant differences from 1hour to 48 hours compared with other groups(P＜0.01),but it was little higher than normal control group at 72 hours with no statistical significance(p＞0.05)2.At 1 hour,the proliferation activity of trophoblastic cells had no statistical significance between hypoxia-reoxygenation group and the three groups of hypoxia-reoxygenation with different concentration Mg²⁺ (p＞0.05).And there was statistical significance between hypoxia-reoxygenation group and hypoxia group(p＜0.05).In addition, from 1hour to 4 hours,the proliferation activity of trophoblastic cells had no statistical significance between hypoxia-reoxygenation group and normal control(p＞0.05),but from 12 to 72 hours,it was lower than normal control(P＜0.05)3.At 1 hour,the proliferation activity of trophoblastic cells in the 3 groups of hypoxia-reoxygenation with different concentration Mg²⁺had no statistical significance compared with hypoxia-reoxygenation group and normal control(p＞0.05).At 4 hours,the cell proliferation activity in the groups of hypoxia-reoxygenation with 1.2mmol/LMg²⁺and with 2.4mmol/LMg²⁺was higher than in hypoxia-reoxygenation group and the p value was less than 0.05,0.01,respectively;while it had no significant difference between hypoxia-reoxygenation with 0.6mmol/LMg²⁺group and hypoxia-reoxygenation group(p＞0.05).From 12 to 72 hours,the cell proliferation activities in the groups of hypoxia-reoxygenation with 0.6mmol/LMg²⁺and with 1.2mmol/LMg²⁺were higher than hypoxia-reoxygenation group,except the group of hypoxia-reoxygenation with 2.4mmol/LMg²⁺.4.The proliferation activity of trophoblastic cells in the groups of hypoxia-reoxygenation with 1.2mmol/LMg²⁺was higher than that in the group with 2.4 mmol/LMg²⁺from 36 to 72 hours,(P＜0.05).The proliferation activity of trophoblastic cells was lower in the group of hypoxia-reoxygenation with 0.6mmol/LMg²⁺than the other two groups with Mg²⁺at 12 hours(P＜0.05),while was higher than these two groups from 48 hours to 72 hours(p＜0.01).The cell proliferation activity was higher in the group of hypoxia-reoxygenation with 2.4mmol/LMg²⁺at 4,12,36 hours(P＜0.01)and had no significant difference compared with hypoxia-reoxygenation group(P＞0.05)Conclutions:1.Hypoxia at earlier period may enhance the proliferation activity of trophoblastic cells.2.The proliferation activity of trophoblastic cells in condition of hypoxia-reoxygenation was lower than that of hypoxia,and was lower than normal control after 12 hours.3.The 0.6～1.2mmol/L Mg²⁺may enhance the proliferation activity of damaged cells caused by hypoxia-reoxygenation,and the effect of 0.6 mmol/L Mg²⁺was more significant.4.The 2.4mmol/L Mg²⁺may reduce the effect of damage on trophoblastic cells which were treated by hypoxia-reoxygenation before 48 hours,but there was no protective effect after 48 hours.