The Study Of Relationship Between The Serum Leptin Concentration And Metabolic Risk Factors In Primary Hypertension Patients

Objective: To explore the relationship between serum leptin levels and major metabolic risk factors in primary hypertension patients.Methods: Seventy-five patients with primary hypertension were enrolled from department of cardiology and endocrinology in the first affiliated hospital of Guangxi Medical University. They were divided into three groups according to the BP grade: hypertension grade 1, hypertension grade 2 and hypertension grade 3. Twenty healthy bodies were assigned as a control group. The height, body weight, blood pressure, waist and hip were measured and BMI and WHR were calculated in all the subject. All objects kept on empty stomach for 12 hours, 5ml fasting venous blood was extracted and centrifugated for 15 minutes under 3000r/min to separate serum. Then the serum was divided into two test tube: one to be examined fasting glucose and serum cholesterol, anther to be stored under -20℃for further examination. Next, all object ate glucose powder 75g and were extract 2ml venous blood for being examined postprandial blood glucose after 2 hours. The serum insulin was measured by specific radioimmunoassay, measurement range is 5.0-160 MIU/ml. The serum concentration for leptin was measured by ELISA method, measurement range is 0.05ng/ml-10μg/ml.Results: The serum leptin concentrations of normal control group, hypertension grade 1, hypertension grade 2 and hypertension grade 3 were 5.62 ±4.14ng/ml, 26.66±14.14ng/ml, 27.32±13.68ng/ml, 32.78±21.35ng/ml respectively. Serum leptin concentrations in hypertension groups were significantly higher than that in normal control group (P<0.001). The level of serum leptin in three groups of primary hypertension did not exist difference obviously, but it was more power, as the BP grade escalated. The statistics results assessed by linear regression analysis showed that the Serum leptin concentration was positively correlated with BMI(r=0.748 , P<0.001), SBP(r=0.682, P<0.001), DBP(r=0.643, P<0.001), FINS(r=0.611, P<0.001).But the serum leptin concentration was not significantly correlated with age, WHR, TG, TC, FBG and PBG. Stepwise regression suggested a regression equation Leptin= - 103.587+0.662×Sex+0.157×BMI+0.331×WHR - 0.312×FBG+0.178×FINS+0.232×SBP.Conclusion: The serum leptin concentrations in primary hypertension are significantly higher than those in normal population. The serum leptin levels are closely correlated with BMI, WHR, FBG and fasting insulin, which suggests high concentration of leptin and major metabolic risk factors are significantly correlated in primary hypertension.