| Objective : By detecting interleukin-15 (IL-15) and interleukin-17 (IL-17) levels in patients with rheumatoid arthritis, we mainly do the following work: (1) to explore the pathogenesis of RA and its relationship with the disease activities; (2) to investigate the role in bone destruction in RA, to reveal pathogenesis of RA further and guide clinical diagnosis and treatment in patients with rheumatoid arthritis (3) to explore the correlativity between IL-15 and IL-17 levels in patients with rheumatoid arthritisMethods: 68 patients with RA( ages 18-65 years old) were outpatients and inpatients in our hospital from March 2006 to February 2007, of which there were 38 cases of activities and 30 cases of remission. And patients enrolled in this study met the American College of Rheumatology(ACR). The kernel index of RA clinical evaluation used the kernel index of RA disease activity evaluation recommended by ACR. We take the right hand X-ray of RA patients and use the American Rheumatism Association RA X-ray phases standards; 32 patients with osteoarthritis(OA) satisfy the primary osteoarthritis of the knee classification standards instituted by American Rheumatism Association in 1986; 30 normal controls were all from the health examinations in our hospital. We detect the levels of IL-15 and IL-17 above four groups with competitive enzyme-linked immunosorbent assay (ELISA).Results : (1)The levels of plasma IL-15 and IL-17 in RA patients were significantly higher than the healthy controls with statistical significance ( p<0.01); The levels of plasma IL-15 and IL-17 in RA patients were significantly higher than that in the OA group with statistical significance (p<0.01); The levels of plasma IL-15 and IL-17 levels were higher than the healthy controls withnot statistical significance (p>0.05). The levels of plasma IL-15 and IL-17 in RA patients with activities was significantly higher than that in RA remission group and the healthy controls with statistical significance (p<0.01); (2) The level of plasma IL-15 in RA patients with activities has negative correlativity with course,morning stiffness,ESR,CRP,RF screening(p<0.01) , has positive correlativity with Ritchie index,DAS,HAQ (p <0.05) ,and has negative correlativity wiyh grip strength (P<0.05). the plasma IL-17 level in RA patients and activities course,pain,the patient comprehensive evaluation were positively correlated (p<0.05), and morning stiffness,Ritchie index,DAS,HAQ,ESR,CRP,RF screening,grip strength were no significant correlation (p>0.05). (3)The levels of IL-15 in RA patients at different X-ray stages were significantly different, furthermore, the levels of IL-15 between phaseâ… and Phaseâ…¡phaseâ…¡and phaseâ…¢phaseâ…¢and phaseâ…£were statistically significant (p<0.01,p<0.01, p<0.05); The levels of IL-17 in RA patients at different X-ray stages were not statistically significant (p>0.05); (4) there was no apparente correlation between IL-15 and IL-17 levels in the plasma of these RA patients (p>0.05)Conclusions: (1) The plasma IL-15 levels in RA patients with activities were significantly higher than that in RA remission group and the healthy controls,and the plasma IL-15 levels have positive correlativity with course,morning stiffness,Ritchie index,HAQ,DAS,ESR,CRP,RF screening , have negative correlativity with grip strength. these suggested that IL-15 play a role in the pathogenesis of RA,and it can be used as a indicator to monitor disease activity . (2) The plasma IL-15 and IL-17 levels in RA were significantly higher than the OA group and the healthy controls. The plasma IL-15 and IL-17 levels in OA were higher than the healthy control group withnot statistical significance. Therefore, IL-15 and IL-17 can be used as indicators to tell RA from OA (3) the plasma IL-15 levels in RA patients at different X-ray stages were of statistical significance. This notes that IL-15 may be involved in the bone destruction in RA. (4) there was no apparente correlation between IL-15 and IL-17 levels in the plasma of RA patients.
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