| Objective:1.Through observing the dynamic changes of VEGF levels in the patients with acuteintracerebral hemorrhage during different phases and studing the relationships among theVEGF levels and the volume of hemorrhage,stroke severity and influential factors(conscious disturbance,high blood press,diabetes,smoking,drinking)in order to explainthe role of VEGF in brain injury and recovery after cerebral hemorrhage.Methods:1.Collected venous blood 4ml from 31 patients with acute intracerebral hemorrhageduring different phases(with in 24,48h,at 3,7 and 14 d after the onset of disease) and 25patients died within 24h once and 12 healthy controls were included.Blood samples were steted in ambient temperature about 30 minutes and centrifuged on3000r/min,then we collected and preserved blood serum at -70℃.2.Serum VEGF level was determined by ELISA.3.All patients were checked on CT scan and 31 patients with acute intracerebralhemorrhage were divided into there group: A group,B group and C group by the volumeof hematoma.4.Stroke severity was evaluated with the Chinese Stroke Scale(CSS) within 24h onadmission. 31 patients with acute intracerebral hemorrhage were divided into there group:the mild group,moderate group and severe group by CSS.Results:1.The levels of VEGF in the patients with acute intracerebral hemorrhage were muchhigher than those in control group(P＜0.001).The levels of serum VEGF graduallyincreased after the onset of acute intracerebral hemorrhage and reached the peak at 7d, and didn't recover at 14d.The 25 patients died within 24 hours had much higher VEGF levelsthan those in control group and the other 31 patients(P＜0.001).2.The serum levels of VEGF during different phases(with in 24,48 h, at 3,7 and14d)have statistically differences between A group and C group(P＜0.001). Within 24,48 hand at 3,7d,there was statistically difference between B group and C group(P＜0.01)and at14 days no statistically differences.Within 48h and at 3,7,14 d, there was statisticalsignificance between A group and B group(P＜0.05) and no statistical significance within24 h.3.The serum levels of VEGF during different phases(with in 24,48,at 3,7 and 14d)have statistically difference between the mild cases and the severe cases(P＜0.001).With in 24,48 h and at 3,7d,there was statistically differences between the moderate casesand the severe cases(P＜0.01)and at 14 days no statistically difference.Within 48 h and at3,7 and 14d,there was statistical significance between the moderate cases and themildcases(P＜0.05)and within 24 hours no statistical significance.4.The volume of hematoma had positive correlation with the scores of neurologicimpairment on admission.The coefficient correlation is equal to 0.916.5.The serum levels of VEGF have dynamic changes following time (P＞0.001). Theconscious state,the history of high blood pressure and diabetes or not,smoking anddrinking or not,all these could not have an effect on the tendency of dynamic changes withthe serum VEGF levels (P＞0.05).Conclusions:1.The levels of VEGF in the patients with acute intracerebral hemorrhage were muchhigher than those in control group and has dynamic changes during different phases.Thelevels of serum VEGF gradually increased after the onset of acute intracerebralhemorrhage and reached the peak at 7d,and didn't recover at 14d.All these meant VEGFparticipated in the process of pathological change after cerebral hemorrhage and it played arole in brain tissue injury and renovation.Conscious state,high blood pressure,diabetes,smoking and drinking could not have an effect on the dynamic changes of the serumVEGF levels.2.The levels of serum VEGF in patients with large hemetoma and high CSS weremuch higher than those in patients with small hemetoma and low CSS.Thecomparison studies of the levels of VEGF with stroke severity and different volume ofhaemorrhage suggested that the raised of serum VEGF levels might be related to theseverity of the disease.
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