Research On The Detection Of The Gene Clusters Associated With PE-DVT By Oligo Microarray

Background Pulmonary embolism (PE) is very common with high morbidity andmortality in clinical practice. Pulmonary embolism (PE) and deep venous thrombosis(DVT),as two diverse developing stages, is a kind of multi-gene, multi-factor disorderdue to abnormal genetics and/or enviroment. It has been proved to be associated withhuge amounts of gene mutations, gene polymorphisms and gene differentialexpressions in human being.Objectives The aim of this study was to find out gene clusters associated with PEthrough the detection of differential expression genes between PE patients andcontrols by oligo microarray, and elucidate the molecular genetics mechanism of PE.Methods The fresh venous blood of 9 PE patients and 33 normal controls werecollected,then the total RNA was extracted and purified.We synthesized 9 PE cRNAprobes labeled with cyanine 3, a standard cRNA probe labeled with cyanine 5 whichwas from total RNA mixture of all the controls.After that,hybridization with AgilentWhole Human Genome Oligo Microarray was performed.Then, the image scanningand data analysis showed the differential expression, which was proved byFluorescence quantitative PCR (FQ-PCR).Results 434 differential expression genes were screened from the 9 microarrays.Of all of the 434 genes,there were 345 genes registered in the Genebank of NCBI,ofwhich 286 genes up-regulated and 59 down-regulated.These gene transcripts areassociated with blood coagulation, immune or inflammatory response,cellproliferation, apoptosis, metabolism, cell growth/maintenance, cell-cell signaling orsignal transduction, cytoskeleton or motility, ion channel or ion transport,transcription, DNA/RNA binding, and so on. 11 genes of them were selected to beamplified by FQ-PCR. The differential expression has been observed in all theselected genes, which is consistent with the microarray.Conclusions Profiling oligo microarray technology is an effective tenique inscreening candidate gene clusters and sensitive genes of PE. The result of theexperiment of microarray was proved reliable by FQ-PCR. The impairment of the balance of blood coagulation system and fibrinolytic system are importantmechanisms for PE. Our study suggests that the inflammatory response, cellproliferation/apoptosis and metabolic disturbance may play a role in the process ofinitiation and development with PE.