The Quantitative Analysis Of The Sperm Images Of Male Infertility

[Objective]The traditional diagnosis of male infertility includes: image of histological cells offers an important way to reveal the biological features of male infertility objectively and accurately. At present, the methods of male infertility diagnosis bases on the observation of morphological structure of sperm cells through microscope. The development of microscopic image processing and analysis technique has made it possible that this technique is widely used in diagnosis of male infertility. The quantitative methods is taking the place of qualitative description step by step, and the objectivity, quantitative and automatization can be realized in diagnosis of male infertility diagnosis. This article mainly discusses morphological structure of sperm cells and their direct effect on the male infertility and its applicability.[Material and methods]1, Individuals: To get the samples from 2005 to 2006 of the urinary surgery, department of gynaecology and obstetrics of the Southern Hospital with a average age of 22 to 47 and health individuals who have normal sexual life. not detected obviously abnormality of didymus. Twenty hundred and sixty seven semen samples were tested. Sperm motility parameters were analyzed by computer aided sperm analysis (CA2SA), and sperm morphology assessed by automated sperm morphology analyzer (ASMA). The cases were classified based on the World Health Organization criteria.2. Methods: put the smear under microscope. Get the pictures inputed into image analysis system. And to detect between two different groups of male infertility. To compare the area and length of tail of sperm cells, test the motility rate, density, antisperm antibody with simple t-test with the level of significance of 0.05.[Results]The observation of the changes of sperm quantity and modality of male after different manganese exposure. VCL, WOB, VAP of the morphologically abnormal group were significantly higher than those of the normal group (P＜0.05, P＜0.001), while MAD, LIN, STR of the abnormal group were significantly lower (P＜0.05, P＜0.001). There were significant positive correlations between the morphologically normal sperm rates and MAD, LIN, WOB, STR, and a significant negative correlation between the morphologically normal sperm rate and ALH. Conclusion: Morphological abnormality of sperm is often accompanied with weak motility, which is probably attributed more to some factors that coact on both sperm motility and morphology than to the influence of sperm morphological abnormality on sperm motility, malignant tumor cells. Morphologically of the sample, less were normal and the other sixty are abnormal. The observation through optical microscope showed that the quantity of the mature sperm decreased remarkably.The result of the experiments indicates that the morphological structure have great relationships of the features of dynamic abnormality. The movement parameters (includes VCL,VAP,ALH,WOB) are extremely higher than the nomal group. But MAD,LIN,STR are lower than the normal group and VSL1 has the trend to decline. The head and neck and middle part of the sperm have lower relationships(r＜0.25).