Study The Fracture Healing Change Of Rabbit Spongy Bone With Denerve Factor

PurposeThis experiment abtains animal models of spongy bone fracture in normal anddenerve condition, analyse the data of change in radioly and pathology, and study therelationship of radiology and pathogy in the course of fracture healingMaterial and methodAdult rabbits(1500+-20 gram), male, randomly devided into two groups, eachgroup contains 8 noes, and called controlled group(group F) and experimentgroup(group NF) respectively. In group F, we produce model of fracture in distalfemoral spongy bone in one leg, use omnipotent material testmachine(DLY-10), according to the calculation and pre-experiment we first exertcompression pressure(950+-50N) to distal femoral bone in single leg to producefracture model, then use improved femoral compression model, take graph ofCT, MR, DWI. so in this way there can be two groups-nerve exist fracture(experimentalgroup) model and normal fracture(contral group), match and compare.after 2 and 4weeks put the animals to death, neutral formaldehyde fixation, analyse AQP4, boneprotection index, MR and CT value. in denerve group, produce fracture model usingfemoral compression model the same above, take graph of CT, MR, DWI. cut off sciatenerve to produce denerved fracture leg. in this way we abtain denerved fracturegroup(experiment group)and normal group(contral group), and match and compare. after2 and 4weeks, take, MR, DWI and put the animal to death, neutral formaldehydefixatin, analyse AQP4, bone protection index, calculate DWI(ADC)valueResult 1,Denerved fracture bone has larger fabric osteotylus, a little fine woven boneformation.2,Osteoprotegerin has little expression on normal bone tissue, Strengthenexpression on fracture healing.It's hightest expression is in the first and seventh day, thistrial indicated that simple fracture group OPG go to peak after one week, and fall sincethis and last weak express, OPG expression in NF groups is lower than F group.3,Aquaporin increase after one dayof surgery, peak at seven day, after that it hasfall off.4,As time goes by, ADC increased in the fracture end, this has statisticalsignificance. P＜0.05. The first weak, the second weak fracture'sADC is lower thannarmal part. The third, forth, fifth weak fracture ADC is highter than normal part. ADC ofF groups is highter than NF groups, P＜0.05.Conclusion1,Denerved factor inhibit sponge bone fracture healing, slow bone formationrate.bone formation quality is pooer than control grope.It is the most obvious at theforth week.2,DWI observed early bone formation is more sensitive than traditionalexamination. It can find early bone formation. high ADC value means higher degree ofhealing, in the denerved group, the increase of ADC value is slow than the control group.3,Aquaporin is a water channel protein, and is related to edema, it increased after1—7 days of surgery and decrease after this. Applying AQP4'sspecial character canheal the problem of local edema in fracture.4,Osteoprotegerin has little expression on normal bone tissue, strengthenexpression on fracture healing. It's hightest expression is seven day, after that it has falloff, OPG encourage osteoblast formation, resistant osteoclast activity. OPG may use infracture healing.