| Pyramidal cells of the frontal cortex and the axon make up of the corticospinal tract (CST) which is the direct central nerve structure to control the limb movement.The direct injurement of CST caused by stroke is the straight nerve structure foundation which leads to the dysfunction of movement (paralysis),hence the research of plasticity of CST is the core problem to research paralysis and rehabilitation mechanism after stroke by acupuncture treatment.In the spinal cord injurement model,the NEP1-40 intrathecal injection shows it can influence the plasticity of CST through adjusting the activity of RhoA signal passway,while whether NEP1-40 is effective to cerebral ischemia patients is not clear.The acupuncture can accelerate the stroke patients rehabilitation,but the machanism is not clear.We take biotin dextran amine(BDA)as nerve tracer to research the plasticity of CST in MCAO model rats,through the transcranial electric stimulation inducing the electric latent period and the amplitude variation to reflect the plasticity of CST function,combined with the"stair test"designed by Montoya to evaluate the snatch ability of paralyzed forelimb, and research the effect of the plasticity of CST in MCAO model rats and rehabilitation by electroacupuncture and NEP1-40 lateral ventricle perfusion.Method:①Choose 36 left-handed rats in the adult SD rats after"stair test"training, and separate them into normal group,sham-operation group, ischemia contrast group,electroacupuncture group,PBS lateral ventricle perfusion group and NEP1-40 lateral ventricle perfusion group,then test the forelimb function on the day before establishing MCAO model,on the 7th and on the 14th and 21th after establishing MCAO model.②The normal group gets no disposal,the sham-operation group just gets the external carotid artery liqation,and the remaining groups get the establishing MCAO model.③inject the 15%BDA into the left first movement cortex area slowly on the 7th day after establishing MCAO models for six group rats.④After injecting BDA, the electroacupuncture group gets the electroacupuncture stimulation at the LI 4 and LI 11 for 15min,and continue 14days,ischemia contrast group gets draught for 15 min like the electroacupuncture group.⑤Take the MEP test after MCAO model on the 21th,take out of the brain and spinal cord tissue and conserve them in the microtherm refrigeratory after perfusion and fixation,observe the CST situation when the BDA is taken by the axon through fluorescence microscope after frost and slice up.Result:①The BDA injected into the left cortex is taken by the axon, and the pyramidal cells and the axon in the left cerebral cortex appear the widespread fluorescence develop,with the time going the BDA taken by the cerebral cortex axon transfers to the midbrain and cervical enlargement along the CST with long distance.②In the NEP1-40 lateral ventricle perfusion group , the CST axon germination amount in the cervical enlargement is much more than the PBS lateral ventricle perfusion group,the neonatal axon crosses the middle line to the other side which means the electroacupuncture stimulation can accelerate the plasticity of CST.③The axon of CST in the MCAO rat intervened by electroacupuncture appears some change like the NEP1-40 lateral ventricle perfusion,the neonatal axon crosses the middle line to the other side which means the electroacupuncture stimulation can accelerate the plasticity of CST.④The compare of snatch ability of left forelimb has no statistic difference on the 1th day before each group rats get establishing MCAO model(P>0.05);the snatch ability of left forelimb in the electroacupuncture group and NEP1-40 lateral ventricle perfusion group is much higher than the cerebral ischemia contrast group on the 14th and 21th day after establishing MCAO model(P<0.05),but lower than PBS lateral ventricle perfusion group and sham-operation group(P<0.05),which means the electroacupuncture and NEP1-40 treatment could help recover the forelimb function for the MCAO rats.⑤on the 21th day after MCAO,the MEP latent period of ischemia contrast group,PBS group,acupuncture group and NEP1-40 group are much longer than the normal group and sham-operation group,the amplitude is obvious low.the compare of MEP latent period and amplitude between the ischemia contrast group and PBS group has no statistic difference;the extention degreement of MEP latent period in acupuncture group is inferior to the ischemia contrast group and PBS group,the differentiation is obvious;The low degreement of MEP amplitude in acupuncture group is inferior to ischemia contrast group and PBS group;the differentiation is obvious;the MEP latent period and amplitude variation trend in NEP1-40 are the same with acupuncture group,the extention degree of MEP latent period is inferior to ischemia contrast group and PBS group;the differentiation is obvious,the low degreement of MEP amplitude in acupuncture group is inferior to ischemia contrast group and PBS group,the differentiation is obvious;all in the above show the electric acupuncture and NEP1-40 treatment can improve the CST function.Conclusion:①Inject the BDA into the cerebral cortex can trace the corticospinal tract effectively.②The"stair test"can reflect the change of snatch function of forelimb for the rats simply , credibly and quantificationally .③MEP can reflex the CST function variation effectively .④NEP1-40 lateral ventricle perfusion can improve the plastivity of CST and snatch function of forelimb for the MCAO rats.⑤The electronic acupuncture treatment can improve the plastivity of CST and snatch function of forelimb for the MCAO rats.All the research conclusion show the NEP1-40 and electronic acupuncture treatment can accelerate the injured nerve rehabilitation through improving the plastivity of CST for cerebral ischemia patients,which shows it is potential to treat the ischemia;the adjustment of electronic acupuncture to the RhoA signal passway activity may be one of machnisms to improve the plastivity of CST and nerve function.
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