The Study Of Adsorption Action Of Purificated Bentonnite From Xinjiang In Pharmacolocy

Objective:Selecting the best maken method of purificated bentonnite;seeing about adsorption ability of purificated bentonnite adsorbing bacterium and studying mechanism of bentonnite adsorbing bacterium simply;studying adsorption action of purificated bentonnite adsorbing uric acid(UAC),urea nitrogen(BUN)and creatinine (CRE)in hyperuricemia chmice blood serum;seeing about adsorption action of bentonnite adsorbing norfloxacin which is used to cure diarrhea usually and adsorption action of bentonnite adsorbing metformin hydrochloride which is used to cure type-Ⅱdiabetes usually.Method:(1)Selecting the best maken method by experiment of time,centigrade and concertration.(2)Selecting active carbon(AC)and bentonnite produced by SIGMA for masculine contrast to study adsorption action of bentonnite adsorbing bacterium in liquid through colony number scale.Quantifying 2g purificated bentonnite,AC and bentonnite produced by SIGMA into Erlenmeyer flask separately and processing sterilization simultaneously,then entering 50ml normal saline into every Erlenmeyer flask under the circumstance of asepsis,then entering 200μl nutrient broth bacterium liquid fostered for 12 hours into every Erlenmeyer flask,placing 2 hours.After 2 hours,imbibing 1ml bacterium liquid from every Erlenmeyer flask into test tube filling 9ml normal saline(regulated pH),mixing uniformity for 1:10 bacterium test solution. Imbibing 1ml 1:10 bacterium test solution into test tube filling 9ml normal saline,mixing uniformity for 1:100 bacterium test solution.Entering bacterium test solution into Petri dish and entering 45～50℃nutrient agar into Petri dish in time,every Petri dish about 15ml.After nutrient agar freezing,overturn Petri dish and foster 24h in 37℃incubator and count colony count.(3)Make chmice hyperuricemia model by hypoxanthine, mensurate content of uric acid(UAC),urea nitrogen(BUN)and creatinine(CRE)in blood serum.Divide 40 chnice into 4 groups randomly,10 chmice every group,select the first group for blank control group,inject 10%hypoxanthine(dose0.1ml/10g)to the later three groups,the second group is model group,the third group is masculine comparison group and the fourth group is bentonnite group.(4)Different dose norfloxacin and metformin hydrochloride and 2.0g bentonnite mixed in 0.1mol/1HCL liquid,place for 2 hours in(37±0.5)℃water and filtrate,mensurate content of norfloxacin and metformin hydrochloride by ultraviolet spectrophotometry(UV).R esult:(1)The absorptivity of purificated bentonnite adsorbing Escherichia coli and staphylococcus aureus is 83.67% and 98.30%separately.(2)Adsorption action of 1.0%bentonnite lipuor adsorbing uric acid(UAC),urea nitrogen(BUN)and creatinine(CRE)in hyperuricemia chmice blood serum is the most strongest,the absorptivity is 49.21%,52.48%and 53.42%separately. (3)The absorptivity of purificated bentonnite adsorbing norfloxacin and metformin hydrochdoride is 99.63%and 99.55%separately.Conclusion:Adsorption action of purificated bentonnite adsorbing bacterium is very strong;reducing action of bentonnite lipuor adsorbing uric acid(UAC),urea nitrogen(BUN)and creatinine(CRE)in hyperuricemia chmice blood serum is evident;otherwise,adsorption action of purificated bentonnite adsorbing norfloxacin and metformin hydrochloride is very strong too.