| Background Parkinson's disease (PD) is the second most common neurodegenerative disorder. With the coming of the old-aged society, the wordwide incidence of PD is rising. According to investigation of Parkinson's Disease Association, there has been up to four millions of PD patients in the world, and our country have 1.72 millions, which occupys the first place. In addition, there is still 100 thousand every year. Therefore, how to deal with PD has become an important medicine and society problem.Researchs have previously demonstrated that Parkinson's disease (PD) is characterized by a relatively selective degeneration of dopaminergic neurons of the substantia nigra pars compacta (SNc) and intracellular proteinaceous inclusions referred to as Lewy bodies. Several studies have suggested that failure of the ubiquitin proteasome system to clear intracellular proteinaceous inclusions may be an important factor in PD. At present, theL-dopamine (L-DA) is wordwide applied to therapy PD. Although it alleviates the symptoms, it can also result in a serises of negative effect, and can't effectively hold back the development of PD. Recently research demonstrates that the effect is significant with Chinese medicine-treated, which not only delays the proccess of PD, but also relieves the negative effect of some chemicals, and can also control other symptoms besides movement disorders. At present, reserchers in our country commomly aimed at the exploration of the anti-oxidation and anti-apoptosis mechanism, However, there is few study on the mechanism of Chinese medicine's therapeutic effect on the ubiquitin proteasome system of PD.Chinese medicine "AnChanLing" has a beneficial effect on treating PD, which has proved by the many year's clinical application. We established the PD rat model of by injecting lactacystin, and observed the AnChanLing effect on the UPS function and anti-apoptosis by immunohistochemistry and TUNEL. We have intention to provide AnChanLing with some experimental evidence on treating PD, and it would be shed light on the therapic mechanism of PD patients as well.Objective To Explore AnChanLing effect on UPS function of PD rat models.Methods we established the rat model of by injecting lactacystin stereotaxically to left side of the mesencephic ventral tegmental area(VTA)and substantian igra pars compactas(SNc) of rats. After 3 weeks, all rats were induced the rotational behavior after injecting apomorphine subcutaneously. 27 successful animals were randomly divided into two groups, model group and Anchanling-treated group. Each group has 10 rats, meanwhile, 10 rats were considered as control group. The control and model groups were treated with physiological saline for 5 weeks, and Anchanling-treated group with Chinese medicine Anchanling decoction. The rotational behavior was evaluated by recording the numer of apomorphine-induced turns once a week. We used tyrosine hydroxylase (TH) immunohistochemical staining to investigate the numbers of dopaminergic neurons in substantia nigra compacta (SNc). TUNEL staining was employed to observe the apoptosis of nerve cells. A-synuclein,ubiquitin positive ceils are applied to elaterate the difference of the UPS function between each group.Results In the contrary of the group of PD rats, the rotational behavior, apoptosic nigral cells of the group of "AnChanLing" treatment were ameliorated effectively. The TUNEL positive cells in the group of Chinese Medcine were significantly less than the model group(P＜0.01), and a-synuclein,ubiquitin positive cells were also less than that.Conclusions AnChanLing could obviously inhibit the apoptosis of neuron cell. It was suggested that AnChanLingcould ameliorate effectively the function of UPS. |
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