| Objective:To investigate the change rule of serum cardiac troponin I (cTnI)level,creatine kinase(CK)and isoenzyme of creatine kinase(CKMB) concentration in wistar rats after adriamycin(ADM)chemotherapy whether undergo vasorel(V)intervention therapy or not before during anaesthetic stage, and to evaluate the influence and protection degree of myocardium injury of wistar rats after ADM chemotherapy with vasorel treatment,and to provide the objective and scientific evidence for anaesthesia,operation safety and cardiac protection during anaesthetic stage in the cancer patients,and to approach deeply the action mechanism of cardiac protection after chemotherapy with vasorel.Methods:Thirty wistar rats were randomly divided into A,B,C 3 groups (n=10),control group(ADM+normal sodium group,group A),experimental group(ADM+V 20mg·kg-1·d-1group,group B and ADM+V 40mg·kg-1·d-1group, group C).All rats were administered intraperitoneally with ADM in equal injections(2.5 mg/kg,each)twice a week over a period of 4 weeks.From the fifth week,the rats in group A were feed with normal sodium injections in ten equal by intragastric administration with equal dosage with V in group B and group C before feeding once a day over a period of one week,the rats in group B and group C were feed with vasorel in ten equal(20mg·kg-1,40mg·kg-1, respectively)before feeding by intragastric administration once a day over a period of one week.All rats were treated with anaesthesia by 2%isoflurane inhalation during anaesthetic stage on the third day after the last administration. (1)The pathological changes of myocardium were observed with light microscope and transmission electron microscope.(2)One ml blood samples were taken from following respectively:before vasorel or normal sodium treatment,before anaesthesia and 20 min after anaesthesia.The samples were centrifuged after they were put aside 30 min,and the blood serum were kept in -80℃refrigerator.(4)Serum cTnI level was assay with MEIA methods,CK and CKMB were assay with colorimetry.(5)All data analyses and statistical procedures were carried out with SPSS 13.0 for Windows,and P＜0.05 in all instances was considered statistically significant.Results:(1)The level of serum CK,CKMB and cTnI in experimental group(group B,group C)before vasorel treatment and in control group(group A)before normal sodium treatment were no statistically significant(P was exceed 0.05),before anaesthesia the level of serum CK,CKMB and cTnI in experimental group were lower than that of control group significantly(P=0.001, P=0.02,P=0.01),and after anaesthesia the level of serum CK,CKMB and cTnI in experimental group were lower than that of control group significantly (P=0.003,P=0.01,P=0.001).(2)In control group,before normal sodium treatment,before anaesthesia and after anaesthesia,the level of serum CK, CKMB and cTnI were statistically significant(P=0.001,P＜0.001,P＜0.001). The level of serum CK,CKMB and cTnI on after anaesthesia were higher than that on before anaesthesia significantly(P=0.001,P=0.001,P＜0.001),the level of serum CK,CKMB and cTnI on after anaesthesia were higher than that on before normal sodium treatment significantly(P=0.001,P＜0.001,P＜0.001), the level of serum CK,CKMB and cTnI on before normal sodium treatment and before anaesthesia was no statistically significant(P=0.07,P=0.62,P=0.16).(3) In experimental group,before vasorel treatment,before anaesthesia and after anaesthesia,the level of serum CK,CKMB and cTnI were statistically significant(P＜0.001,P=0.001,P=0.001).the level of serum CK,CKMB and cTnI on before anaesthesia were lower than that before vasorel treatment significantly(P＜0.001,P=0.001,P=0.001),the level of serum CK,CKMB and cTnI after anaesthesia were also lower than that before anaesthesia significantly (P=0.001,P=0.001,P＜0.001),but the level of serum CK,CKMB and cTnI after anaesthesia were higher than that before anaesthesia significantly(P=0.001, P＜0.001,P=0.002).(4)Before vasorel treatment,before anaesthesia and after anaesthesia,in group B and group C,the level of serum CK,CKMB and cTnI were no statistically significant(p value was exceed 0.05).(5)The pathological changes of myocardium were significantly observed with light microscope in control group,there were severely cellular degeneration and necrosis,but there were slightly cellular degeneration and necrosis in experimental group.The pathological score of myocardium was significant difference in experimental group and control group(P=0.001),the score of group B was higher than that of group A and group C(p=0.01,p=0.03),but group B to group C was no statistically significant(P=0.06).(6)The changes of myocardium ultramicrostructure: In control group,muscular fibrils were thinningzed,breakaged, dissolved and agglutinated,sarcoplasmic reticulum were dilated and vacuolus, the most mitochondrium were swelling.In experimental group,the above changes were slighter than control group,part of mitochondrium were swelling, sarcoplasmic reticulum were dilated slightly.Conclusions:(1)The level of serum CK,CKMB and cTnI of wistar rats after ADM chemotherapy were increased,and they were advanced during anesthetic stage,it is impossible that isoflurane anesthesia may induce the aggravation of adriamycin-induced cardiac injury.(2)The level of serum CK, CKMB and cTnI of wistar rats after ADM chemotherapy were decreased with vasorel treatment,with during anaesthetic stage,it indicated that vasorel may restrain myocardial damage after ADM chemotherapy and it protect myocardium during anaesthesia stage.(3)Serum cTnI was one of the sensitive biomarkers of cardiac injury during anesthetic stage,it was more sensitive and specific than CK and CKMB.
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