| ObjectiveTo establish the allogeneic animal bone marrow and orthotopic liver transplantation model in rat and explore the mechanisms of tolerance induced by chimera, investigate the method and feasibility for enhance the stability and prolongation of the survival time of liver transplantation model in rat.MethodsSD rats(donor,♂) and Wistar rats(recipient,♀) were conditioned by three methods: Groupâ… orthotopic liver transpIantations in rats were performed using modified Kamada's two-cuff technique; Groupâ…¡Wistar rats(recipient,♀)were induced with sublethal total body irradiation (TBI, 11Gy) and groupâ…¢with TBI(7Gy), followed by infusion of SD (donor,♂) rat bone marrow cells(8x10~7) within 4h, then groupâ…¢were administered cytoxan (CTX, 50mg/kg) intraperitoneally 2 days later only. According to the GenBank, the specific primer of rat SRY gene was designed. Recipient rats were detected for donor origin cells in the peripheral blood lymphocyte on day 10, 20 by polymerase chain reaction(PCR). The PCR product was analyzed by electrophoresis. Then perform orthotopic liver transplantations in rats 28 days later. To explore tolerance mechanism through delayed typehypersensitivity (DTH) and survival time after liver transplantation in the rats.ResultsChimera of SD rats can be found in the peripheral blood lymphocyte of the tolerance Wistar rats in Groupâ…¡andâ…¢. Wistar rats were specifically tolerant to the SD rats in DTH. Groupâ… rats died after 4-5 days, Groupâ…¡andâ…¢the survival rate was 80% and 84.2% after 1 week, But wistar rats in groupâ…¡have worse survivals compared with that in groupâ…¢.ConclusionsTreatment of 7Gy TBI and the injection of CTX(50 mg/kg) plus donor bone marrow transplantation (BMT) can successfully establish rat chimera model and enhance the survival time of liver transplantation model in rat.Infusion of the bone marrow cells might be an effective method for maintaining the tolerant state and for the prolongation of the survival time of liver transplantation model in rat. |
